番荔枝内酯三种不同提取方法的比较

目的 比较不同提取方法从光叶番荔枝种子中提取番荔枝总内酯的得率.HT5"H方法 以番荔枝总内酯及具有显著抗肿瘤活性的番荔枝内酯单体成分Squamocin提取率为考察指标,以HPLC及比色法为含量测定方法,比较不同提取方法所得总内酯及Squa mocin的含量.结果 醇浸渍、水浸渍醇渗漉和超临界CO2萃取法所得总内酯在原药材中含量分别为2.28%,3.59%,17.85%,Squamocin含量分别为0.1467%,0.1307%,0.1138%.结论 超临界CO2萃取番荔枝内酯得率较溶剂提取法高,且操作简单,为较好的提取方法.

Comparison of Three Different Extraction Methods of Annonaceous Acetogenins

OBJECTIVE Study on the yield of annonaceous acetogenins from th e Seed of Annona glabra Linn with different extraction methods.METHODS Using the methods of HPLC and chromatometry as determinations and extr action yields of total annonaceous acetogenins and monomer composition Squamocin having significant anti-tumor a ctivity as target,Comparing the contents of total annonaceous acetogenins and Squamocin with different extractions.RESULTS Alcohol impregnation,Alcohol soaking water Percolation and sup ercritical CO2 extraction from the original herb total annonaceous acetogenins contents are 2.28%.3.59% and 17.85%,Squamocin contents are 0.1467%.0.1307% and 0.1138%.CONCLUSION Supercritical CO2 extraction Ann onaceous acetogenins yield more than solvent extraction,and the operation is si mple,for better extraction methods.