幽门螺杆菌对胃癌细胞RhoA表达和活性的影响

目的: 研究幽门螺杆菌(Helicobacter pylori, Hp)对胃癌细胞中的RhoA的表达和活性的影响,探讨Hp与胃癌之间的相互关系.方法: Hp由人胃黏膜组织中分离,固体培养,收获后用超声波方法裂解Hp,以裂解液刺激胃癌细胞SGC-7901,用pull-down 方法来获得细胞裂解液中的活性RhoA,用Western blot方法来检测细胞裂解蛋白中的活性RhoA的变化,用逆转录聚合酶链反应(RT-PCR)方法半定量法测定RhoA mRNA相对量.结果: 用Hp裂解液刺激细胞10 min,总RhoA无明显变化,而活性RhoA升高.结论: Hp裂解液能增加胃癌细胞中活性RhoA的表达,呈剂量-效应关系,但总RhoA无明显变化,它可能是参与胃癌细胞的迁移.

Expression and ctivity of RhoA in the gastric cancer cells stimulated by helicobacter pylori

Objective: The experiment was designed to study the effect Helicobacter pylori(Hp) on the activity of RhoA in the gastric cancer cells and investigate the relation between Hp and gastric cancer.Methods: Hp was isolated from human gastric mucosa and cultured on solid culture plate.The lysate of Hp was obtained by sonication.After stimulating the gastric cancer cell line SGC-7901 for ten minutes by the lysate of Hp,we obtained the active RhoA by pull-down assay and examined the level of active RhoA by western blot.The mRNA level of RhoA gene was examined by semi-quantitative RT-PCR. Results: In the gastric cancer cells stimulated by the lysate of Hp for ten minutes,the level of active RhoA was increased but the mRNA level did not change. Conclusion: The lysate of Hp could stimulate the expression of active RhoA dose-dependently,but the expression of RhoA was not increased,it may participate the migration of the gastric cancer cells.