| Vc／FeSO4诱发微粒体脂质过氧化模型的建立 |
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| 引用本文： | 李富君 孙贵范. Vc／FeSO4诱发微粒体脂质过氧化模型的建立[J]. 中国卫生检验杂志, 1997, 7(2): 73-75 |
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| 作者姓名： | 李富君 孙贵范 |
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| 作者单位： | 中国医科大学卫生系劳动卫生教研室(李富君，孙贵范)，中国医科大学卫生系中心实验室!110001(赵明)，中国医科大学癌症检测中心(刘淑兰，吕秀强，陆春伟) |
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| 摘 要： | 运用体外实验、对Vc／FeSO_4。诱发的微粒体脂质过氧化（LP）发生条件及反应历程进行了探讨。结果表明：在37℃水浴30min，微粒体蛋白浓度为1.0mg／ml，4μmol／LFeSO_4.pH值7．4的0．1mol／LTris－HCl缓冲浪的条件下，Vc低浓度（0.25mmol/L以下）时可刺激LP，但高浓度的Vc（＞0.25mmol/L）则抑制LP，其中Vc为0．05mmol/L时．微粒体中MDA含量于反应后迅速上升，30min达到高峰.以后趋于平稳。揭示：一定比例的Vc／FeSO_4可有效地激发微粒体LP。本研究初步建立了微粒体LP模型。
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| 关 键 词： | Vc 微粒体模型 脂质过氧化 |
The foundation of microsomal Lipid Peroxidation model induced by Vc/FeSO_4 |
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| Li Fu Jun, Sun Gui Fan, et al. The foundation of microsomal Lipid Peroxidation model induced by Vc/FeSO_4[J]. Chinses Journal of Health Laboratory Technology, 1997, 7(2): 73-75 |
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| Authors: | Li Fu Jun Sun Gui Fan et al |
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| Affiliation: | Department of Preventive Medicine. China Medical University Shen yang 110001 |
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| Abstract: | The condition and the time course of lipid peroxidation (LP)induced by Vc/FeSO4 in rat hepatic microsomes in vitro was studied. The results revealed that when microsomes (1. 0mg protein/ml) were incubated with 4umol/LFeSO4at 37 C for 30 min in pH7. 4,O. lmol/L, Tris-HCl buffer, Vc, at lowconcentrations(<0.25mmol/L ) stimulated LP.but at higher concentration (>0.25mmol/L),Vc inhibited LP. The content of Malonaldehyde (MDA) rised rapidly after addition of 0.05mmol/L Vc and 4umol/L, FeSO_4 O4, reached maximal levels in 30min, very litle additional LP occured over the next two hours. The rcsullt suggested that Ve/FeSO_4 on the appropriate scale could induced ellectively microsomal LP. A model induced by Vc/FeSO4 was founded preliminarily in our experiment. |
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| Keywords: | Vc microsomal model Lipid peroxidation |
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