| 结直肠癌中RhoC、PRL-3与FMNL2的表达及其意义 |
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| 引用本文： | 罗皓,李靖宇,朱震威,石丰榕,余力. 结直肠癌中RhoC、PRL-3与FMNL2的表达及其意义[J]. 热带医学杂志, 2013, 0(3): 271-273,F0004 |
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| 作者姓名： | 罗皓 李靖宇 朱震威 石丰榕 余力 |
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| 作者单位： | 南方医科大学附属珠江医院病理科,广东广州510280 |
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| 基金项目： | 广东省自然科学基金（9151503101000016） |
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| 摘 要： | 目的探讨RhoC、PRL-3及FMNL2在结直肠癌(CRC)中的表达及其相互作用关系。方法利用免疫组化、Western blot检测在不同结直肠癌细胞中RhoC、PRL-3蛋白含量变化;用Realtime-RTPCR法分析RhoC、PRL-3mRNA表达情况。结果免疫组化显示FMNL2基因沉默后,RhoC的平均光密度值(AOD)明显下降,而PRL-3的AOD值未见明显差异。随着结直肠癌细胞中FMNL2蛋白水平下调,RhoC蛋白水平表达明显下降,RhoC mRNA的表达水平是SW620细胞的0.618倍,差异有统计学意义(P0.05),而PRL-3蛋白含量及mRNA的表达水平均未见明显差异。结论 FMNL2可能参与RhoC调控转移机制,而FMNL2对PRL-3的表达可能无反馈性调节作用。
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| 关 键 词： | 结直肠癌 RhoC PRL-3 FMNL2 |
Expression of RhoC, PRL-3 and FMNL2 in colorectal cancer |
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| LUO Hao,LI Jing-yu,ZHU Zhen-wei,SHI Feng-rong,YU Li. Expression of RhoC, PRL-3 and FMNL2 in colorectal cancer[J]. Journal Of Tropical Medicine, 2013, 0(3): 271-273,F0004 |
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| Authors: | LUO Hao LI Jing-yu ZHU Zhen-wei SHI Feng-rong YU Li |
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| Affiliation: | (Department of Pathology, Affiliated Zhujiang Hospital of Southern Medical University, Guangdong, Guangzhou 510280, China) |
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| Abstract: | Objective The aim of this study was to investigate the expression of RhoC and PRL-3 proteins in FMNL2 silenced colorectal carcinoma （CRC）. Methods The protein expression of RhoC, PRL-3 and FMNL2 was detected by immunohistochemistry in different human colorectal cancer cell lines. The mRNA and protein expression was detected by real- time RT-PCR and Western blot, respectively. Results Results from immunohistochemical staining showed that the expression of RhoC was significantly decreased in FMNL2 silenced SW620 cell lines. However, the expression of PRL-3 was not changed. Conclusion The expression of RhoC might be regulated by FMNL2 and RhoC might promote the distant metastasis of cancer cell by FMNL2. |
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| Keywords: | colorectalcancer RhoC PRL-3 FMNL2 |
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