| 野生与栽培苦参生物碱含量的比较研究 |
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| 引用本文： | 姚丽,易红,高慧敏,马蕙文,张继远,田莲超,刘晓谦,王智民.野生与栽培苦参生物碱含量的比较研究[J].中国中药杂志,2016,41(21):3968-3974. |
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| 作者姓名： | 姚丽 易红 高慧敏 马蕙文 张继远 田莲超 刘晓谦 王智民 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;广东药科大学 中药学院, 广东 广州 510006,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700,中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700 |
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| 基金项目： | 中药饮片质量保障体系研究项目（201507002）；国家“重大新药创制”科技重大专项（2012ZX09103201-009，2012ZX09103201-027）；中国中医科学院客座研究员联合创新研究项目（ZZ070837） |
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| 摘 要： | 为比较苦参药材的野生品与栽培品在生物碱成分含量上的质量差异,对收集的22个栽培样本和17个野生样本,用HPLC同时测定了6种生物碱(氧化苦参碱、氧化槐果碱、槐定碱、N-甲基野靛碱、苦参碱、槐果碱)的含量,以独立样本t检验,辅以聚类分析(HCA)和主成分分析(PCA)方法进行评价。独立样本t检验表明:野生品与栽培品在N-甲基野靛碱、苦参碱、槐果碱3种成分含量上有差异(苦参碱及槐果碱P0.05,N-甲基野靛碱P0.01),氧化苦参碱、氧化槐果碱、槐定碱野含量无差异,但组内差异均较大;聚类分析及主成分分析表明:野生品与栽培品在生物碱含量上无差异。故认为栽培品与野生品在生物碱含量方面无差异。
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| 关 键 词： | 苦参 野生与栽培 生物碱 |
| 收稿时间： | 8/1/2016 12:00:00 AM |
Comparison of alkaloids in roots of cultivated and wild Sophora flavsecens |
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| YAO Li,YI Hong,GAO Hui-min,MA Hui-wen,ZHANG Ji-yuan,TIAN Lian-chao,LIU Xiao-qian and WANG Zhi-min.Comparison of alkaloids in roots of cultivated and wild Sophora flavsecens[J].China Journal of Chinese Materia Medica,2016,41(21):3968-3974. |
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| Authors: | YAO Li YI Hong GAO Hui-min MA Hui-wen ZHANG Ji-yuan TIAN Lian-chao LIU Xiao-qian and WANG Zhi-min |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Ingredient Enrichment of Traditional Chinese Medicine, Beijing 100700, China |
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| Abstract: | To compare the contents of alkaloids in theroots of cultivated and the wild Sophora flavsecens, 22 cultivated and 17 wild samples were collected. HPLC method was employed to simultaneously determine the contents of six alkaloids(oxymatrine, oxysophocarpine, sophoridine, N-methylcytisine, matrine, and sophocarpine). Independent t-test, hierarchical clustering analysis(HCA) and principal components analysis(PCA) were applied to analyze and evaluate the cultivated and the wild S.flavsecens. With a great wide range of the inter-group, the t-test results showed that the contents difference of N-methylcytisine, matrine, and sophocarpine were statistical significance(matrineandsophocarpine P<0.05, N-methylcytisine P<0.01).However, it was not statistically significant for oxymatrine, oxysophocarpine, and sophoridine.HCA and PCA showed that there were no significant differences in the contents of alkaloids of cultivated and wild S. flavsecens. The results indicated that there were no differences in the contents of alkaloids of cultivated and wild S. flavsecens. |
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| Keywords: | Sophora flavsecens cultivated and wild alkaloid |
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