| Stable clonal expansion of the T-cell receptor Vβ6， Vβ17 and Vβ19 T cells in a cGVHD case using genescan analysis |
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| 作者单位： | Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China;Department of Hematology, Guangdong Provincial People’s Hospital, Guangzhou 510080, China;Department of Hematology, First Affiliated Hospital of Medical College, Jinan University, Guangzhou 510632, China;Department of Hematology, First Affiliated Hospital of Medical College, Jinan University, Guangzhou 510632, China;Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China;Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China |
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| 摘 要： | Objective To investigate the distribution and clonality of the T-cell receptor (TCR) Vβ repertoire in chronic graft versus host disease (cGVHD).Methods The complementarity determining region 3 (CDR3) of the TCRβ gene with 24 variab le regions was amplified in peripheral blood mononuclear cells drawn from one cG VHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 o r 45 months respectively, using RT-PCR, to observe the expression of TCR Vβ re pertoire T cells.The PCR products were further analyzed by genescan to evaluat e clonality of T cells.Results Fourteen or 16 TCR Vβ subfamily T cells were detected in each sample of cGVHD c ase. Oligoclonal T cells were identified in TCR Vβ 6， 16， 17， 19 and 21 subfamili es.The stable clonal T cells in all samples were identified in Vβ6, Vβ17 and Vβ21 subfamilies. Conclusion Skewing distribution and stable clonal expansion of T cells can be found in cG VHD cases and it may be related to the initiation of cGVHD.
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| 关 键 词： | TCR Vβ gene genescan T cell clonality graft versus host disease |
Stable clonal expansion of the T-cell receptor V beta 6, V beta 17 and V beta 19 T cells in a cGVHD case using genescan analysis. |
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| LI Yangqiu,DU Xin,XU Minhua,ZHANG Tao,YANG Lijian,CHEN Shaohua. Stable clonal expansion of the T-cell receptor V beta 6, V beta 17 and V beta 19 T cells in a cGVHD case using genescan analysis.[J]. Chinese medical journal, 2001, 114(5): 489-492 |
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| Authors: | LI Yangqiu DU Xin XU Minhua ZHANG Tao YANG Lijian CHEN Shaohua |
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| Affiliation: | Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China. jnyanqiuli@163.net |
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| Abstract: | OBJECTIVE: To investigate the distribution and clonality of the T-cell receptor (TCR) V beta repertoire in chronic graft versus host disease (cGVHD). METHODS: The complementarity determining region 3 (CDR3) of the TCR beta gene with 24 variable regions was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the expression of TCR V beta repertoire T cells. The PCR products were further analyzed by genescan to evaluate clonality of T cells. RESULTS: Fourteen or 16 TCR V beta subfamily T cells were detected in each sample of cGVHD case. Oligoclonal T cells were identified in TCR V beta 6, 16, 17, 19 and 21 subfamilies. The stable clonal T cells in all samples were identified in V beta 6, V beta 17 and V beta 21 subfamilies. CONCLUSION: Skewing distribution and stable clonal expansion of T cells can be found in cGVHD cases and it may be related to the initiation of cGVHD. |
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