| HPLC测定丹葛胶囊剂中葛根素的含量 |
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| 引用本文： | 黄平,陶秀华,邾枝花.HPLC测定丹葛胶囊剂中葛根素的含量[J].安徽卫生职业技术学院学报,2012(5):83-84. |
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| 作者姓名： | 黄平 陶秀华 邾枝花 |
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| 作者单位： | [1]安徽中医学院药学院,合肥230031 [2]安徽医学高等专科学校,安徽230601 [3]安徽省中药制剂工程技术研究中心,安徽230031 |
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| 基金项目： | 安徽高校省级自然科学研究重点项目资助（NO：KJ2010A210） |
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| 摘 要： | 目的:建立高效液相色谱法测定丹葛胶囊剂中葛根素含量的测定方法。方法:色谱柱为Hypersil ODS(4.0mm×250mm,5μm);以甲醇-水-醋酸(25:75:1)为流动相;流速:1.0ml/min;检测波长:270nm进行实验。结果:线性回归方程为Y=1.738×106X+4404.5,相关系数r=0.9998,葛根素在0.050~2.00μg范围内呈线性,平均回收率为99.83%,RSD为1.52%。结论:本方法准确可靠,稳定性、重现性好,适用于丹葛胶囊剂的含量测定。
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| 关 键 词： | 丹葛胶囊 葛根素 HPLC 含量测定 |
Determination of neogambogic acid in Furong Gel by HPLC |
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| HUANG Pin,TAO Xiu-hua,ZHU Zhi-hua.Determination of neogambogic acid in Furong Gel by HPLC[J].Journal of Anhui Heaith Vocational & Technical College,2012(5):83-84. |
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| Authors: | HUANG Pin TAO Xiu-hua ZHU Zhi-hua |
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| Institution: | 1.Anhui University of Traditional Chinese Medicine,Hefei 230031,Anhui2.Anhui Medical College,Hefei 230601,Anhui |
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| Abstract: | Objective： To establish a HPLC method for determinating the content of puerarin in Dange capsules.Methods： The determination of puerarin Dange capsules was carried on a column of Hypersil ODS（4.0×250mm,5μm） by HPLC,with the mixture of methanol-water-glacial acetic acid（25∶75∶1） as the mobile phase;the flow rate was 1.0 ml/min-1;the detection wavelength was 270nm.Results： The linear range of determination by HPLC was 0.050~2.00μg（r=0.9998）.The precision（RSD） was 1.52%.The average recoveries was 99.83%.Conclusion： The method is accurate and reliable,which is suitable for the determination of puerarin in Dange capsules. |
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| Keywords: | Dange capsules puerarin HPLC determination |
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