| 冬虫夏草中游离麦角甾醇的研究 |
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| 引用本文： | 石岩,肖新月,程显隆,魏锋,赵英永,林瑞超.冬虫夏草中游离麦角甾醇的研究[J].中药研究与信息,2012(7):15-17. |
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| 作者姓名： | 石岩 肖新月 程显隆 魏锋 赵英永 林瑞超 |
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| 作者单位： | [1]中国食品药品检定研究院,北京100050 [2]西北大学陕西省生物医药重点实验室,陕西西安710069 |
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| 摘 要： | 目的：建立冬虫夏草药材中麦角甾醇的RP—HPLC含量测定方法。方法：冬虫夏草药材粉末经甲醇超声提取后，用RP—HPLC测定。采用Phenomenex Luna C18（250mm×4．6mm，5μm）色谱柱，流动相为甲醇-水（98：2）；流速为1mL·min^-1；检测波长为282nm。结果：麦角甾醇进样量在0．04272～0．4272μg与峰面积呈良好的线性关系（r=0．9999）；麦角甾醇的平均回收率为98．7％（n=6）。结论：采用建立的RP-HPLC方法测定冬虫夏草药材中麦角甾醇的含量，方法灵敏、快速、准确。
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| 关 键 词： | 冬虫夏草 含量测定 反相高效液相色谱法 质量控制 麦角甾醇 |
Study of Ergosterol in Cordyceps |
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| SHI Yan,XIAO Xin-yue,CHENG Xian-long,WEI Feng,ZHAO Ying-yong,LIN Rui-chao.Study of Ergosterol in Cordyceps[J].Research & Information on Traditional Chinese Medicine,2012(7):15-17. |
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| Authors: | SHI Yan XIAO Xin-yue CHENG Xian-long WEI Feng ZHAO Ying-yong LIN Rui-chao |
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| Institution: | 1. National Institutes for Food and Drug Control ,Beijing 100050 ,China; 2. Biomedicine Key Laboratory of Shanxi Province,Northwest University,Xi＇an 710069, China) |
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| Abstract: | Objective： To establish a RP-HPLC method for quantitative analysis of ergosterol in Cordyceps. Methods：A Phenomenex Luna C18（250 mm ×4. 6 mm,5 μm） column was used with the mobile phase being methanol-water （ 98 ： 2 ）, flow rate being 1.0 mL·min ^- 1, detecting wavelength being 282 nm . Results： The method had good linear relationship within the range of 0. 042 72 - 0. 427 2 μg （ r = 0. 999 9 ）. The average recovery was 98.7% （n = 6）,RSD =4. 5%. Conclusion：The method is sensitive,quick and accuracy. |
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| Keywords: | Cordyceps Determination RP-HPLC Quality control Ergosterol |
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