朝藿定C对去卵巢小鼠雌激素的影响

目的：观察朝藿定C对去卵巢小鼠雌激素的影响。方法：以0．4％戊巴比妥钠麻醉动物后，造模小鼠切除双侧卵巢，对照小鼠切除卵巢旁同样大小的脂肪团。术后45d将小鼠分为6组：假手术组（等容积常水）、模型组（等容积常水）、阳性药组（己烯雌酚2mg·kg-1）、朝藿定c高剂量组（20mg·kg-1）、朝藿定C中剂量组（10mg·kg-1）、朝藿定C低剂量组（5mg·kg-1），每组14只小鼠。各组动物按各自药物和剂量灌胃给药，每日1次。连续给药60d后，处死小鼠，取子宫称重，计算子宫系数，用ELISA方法测定血清雌二醇的含量。结果：与对照组比较，模型组小鼠体重明显增加，子宫重量、子宫系数及雌二醇含量则显著降低；与模型组比较，朝藿定C各剂量组及己烯雌酚组小鼠体重明显减轻，子宫重量、子宫系数及雌二醇含量则显著增加（P〈0．01或P〈0．05）。结论：朝藿定C具有雌激素样作用。

Effect of Epmedin C on Estrogen in Ovariectomized Mice

Objective： To observe the effect of Epmedin C of estrogen in ovariectomized mice. Methods： Anesthetized with 0. 4% pentobarbital sodium, mice of the ovariectomized groupwere conducted with Bilateral oophorectomy, while fat beside ovaries were removed on mice of the sham-operation group. After operation 45days, all mice were divided into 6 groups, as sham-operation group （isometric water）, model group （isometric water）, positive drug group （diethyl-stilbestrol, 2 mg·kg-1）, high doses of Epmedin C group（20 mg·kg-1）, middle doses of Epmedin C group（10 mg·kg-1）, low doses of Epmedin C group（5 mg·kg-1）, with 14 mice in each group. Each group underwent intragastric administration once a day for 60 days. Then all mice were killed to weigh the uterus and calculate uterine factor. The content of estradiol in serum was determined by ELISA. Results： Compared with the sham-operation group, body weight of mice of model Stoup were significantly increased, uterus weight and uterine factor and estradiol levels were significantly reduced, which suggested a significant difference. In comparison of the ovariectomized group, body weight of mice were relieved significantly and uterus weight and uterine factor and estradiol levels were increased significantly in all Epmedin C groups and diethylstilbestrol group （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： Epmedin C has estrogen-like effects for ovariectomized mice.