乳香中三萜类成分的薄层色谱鉴别和含量测定

目的：以乳香中主要三萜类成分为指标，建立和完善乳香的定性定量分析方法。方法：用TLC鉴别法，以三萜酸类成分11-羰基-β-乙酰-乳香酸（AKBA）为对照品，采用硅胶GF254薄层板，以环己烷-乙酸乙酯-冰醋酸（10：2：0．2）为展开剂，鉴别乳香药材中AKBA；用HPLC测定方法，Agilent TC-C18色谱柱（250mm×4．6mm，5μm），乙腈-0．05％磷酸溶液（80：20）为流动相，流速：0．8mL·min^-1，检测波长：250nm，测定不同来源乳香药材中AKBA和11-羰基-β-乳香酸（KBA）含量。结果：乳香TLC图谱中，AKBA斑点清晰、分离效果好；HPLC含量测定法中，AKBA和KBA分别在0．02463～0．2463mg·mL^-1和0．01004～0．1004mg·mL^-1，线性关系良好。AKBA和KBA的回收率分别为96．1％（RSD=3．1％）和96．4％（RSD=3．5％）。结论：所建立的TLC和HPLC法操作简便、结果准确、重复性好，可作为乳香定性、定量的质量控制方法。

Qualitative and Quantitative Analysis of Triterpenes in Olibanum by TLC and HPLC

Objective：To establish and improve the qualitative and quantitative analysis of triterpenes in the Olibanum. Methods： TLC was used for qualitative analysis of triterpenes in the Olibanum, silica gel GF254, mixed with sodium carboxymethyl cellulose as the coating substance and cyclohexane-ethyl acetate-glacial acetic acid （10：2：0. 2）as mobile phase,and triterpene acids composition of Olibanum as reference substance namely acetyl-11-keto-β-boswellic acid （AKBA）. HPLC method was used for quantitative analysis of AKBA and 11 -keto-β-boswellic acid （ KBA）, using Agilent TC-C 18 column（250 mm ×4. 6 mm ,5 μm） . The mobile phase was a mixture of acetonitrile-0.05 % phosphoric acid（80：20） . The flow rate was 0. 8 mL-min-1. The column temperature was 30 ℃. The detection wavelength was 250 nm. Results：The TLC method had high sensitivity and showed clear spots of AKBA. The recoveries of AKBA and KBA by HPLC method were 96. 1% （ RSD = 3.1% ） and 96.4% （ RSD = 3.5% ）, respectively. Conclusion： TLC and HPLC methods are simple, accurate and repeatable. The methods can be used for the quality control of Olibanum.