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北沙参种子发芽和生活力检验方法的研究
引用本文:郝江波,李欧,胡璇,李卫东.北沙参种子发芽和生活力检验方法的研究[J].中药研究与信息,2012(5):29-31.
作者姓名:郝江波  李欧  胡璇  李卫东
作者单位:[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学中药材规范化生产教育部工程研究中心,北京100102
基金项目:[基金项目]国家科技重大专项项目(2009ZX09308-002)
摘    要:目的:确定北沙参种子适宜的发芽和生活力的检验方法。方法:采用不同的发芽床,放置于不同的温度下培养,观察不同条件对北沙参发芽率的影响,确定适宜的发芽条件;采用四唑法(TTC)对北沙参着色率进行检验,设计不同浓度和不同温度的正交试验,通过对不同条件下北沙参染色情况的观察,确定适宜的生活力检验方法。结果:北沙参以25℃,滤纸作为发芽床为适宜发芽条件;以30℃,0.6%TYC浓度,染色时间5h为适宜的生活力检验方法。结论:通过对北沙参发芽和生活力检验方法的研究,为其种子检验规程和质量标准的制定提供依据。

关 键 词:北沙参  种子  发芽  生活力  检验方法

Testing Methods of Germination and Viability of Glehnia littoralis Fr. Schmidt ex Miq. Seed
HAO Jiang-bo,LI Ou,HU Xuan,LI Wei-dong.Testing Methods of Germination and Viability of Glehnia littoralis Fr. Schmidt ex Miq. Seed[J].Research & Information on Traditional Chinese Medicine,2012(5):29-31.
Authors:HAO Jiang-bo  LI Ou  HU Xuan  LI Wei-dong
Institution:1. School of Chinese Pharmacy,Beijing University of Chinese Medicine ,Beifing 100102, China ; Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs of Education Ministry, Bering University of Chinese Medicine , Beijing 100102, China)
Abstract:Objective: To determine the suitable testing methods of germination and viability of Glehnia littoralis Fr. Schmidt ex Miq. seed. Methods: The appropriate seed germination conditions were obtained by testing the influence of the different germination beds and temperatures on the average germination rate. The appropriate testing method of seed viability was found out by observing the effects of different concentration and temperature of TCC on the seed dye rate. Results: The appropriate conditions for seed germination are at 25 ℃ on filter paper. The best testing conditions of seed viability are dying for 5 hours in0. 6% TCC at 30℃ Conclusion: Based on the research of germination conditions and testing method of seed viability, our study offers help for the establishment of testing rules and quality standard of G. littoralis seed.
Keywords:Glehnia littoralis Fr  Schmidt ex Miq    Seed  Germination  Viability  Testing methods
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