Semi-automatic detection of myocardial trabeculation using cardiovascular magnetic resonance: correlation with histology and reproducibility in a mouse model of non-compaction |
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Authors: | Julien Frandon Stéphanie Bricq Zakarya Bentatou Laetitia Marcadet Pierre Antoine Barral Mathieu Finas Daniel Fagret Frank Kober Gilbert Habib Monique Bernard Alain Lalande Lucile Miquerol Alexis Jacquier |
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Affiliation: | 1.Aix-Marseille University, CNRS, CRMBM,Marseille,France;2.Department of Radiology,Timone University Hospital,Marseille,France;3.Department of Radiology,N?mes University Hospital,N?mes,France;4.Le2i,Université de Bourgogne Franche-Comté,Dijon,France;5.CNRS UMR 7288, Developmental Biology Institute of Marseille,Aix-Marseille University,Marseille,France;6.INSERM, U1039, Radiopharmaceutiques Biocliniques,Université Grenoble Alpes,Grenoble,France;7.Department of Cardiology, APHM,la Timone Hospital,Marseille,France;8.Department of MRI,University Hospital Francois Mitterrand,Dijon,France |
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Abstract: | BackgroundThe definition of left ventricular (LV) non-compaction is controversial, and discriminating between normal and excessive LV trabeculation remains challenging. Our goal was to quantify LV trabeculation on cardiovascular magnetic resonance (CMR) images in a genetic mouse model of non-compaction using a dedicated semi-automatic software package and to compare our results to the histology used as a gold standard.MethodsAdult mice with ventricular non-compaction were generated by conditional trabecular deletion of Nkx2–5. Thirteen mice (5 controls, 8 Nkx2–5 mutants) were included in the study. Cine CMR series were acquired in the mid LV short axis plane (resolution 0.086?×?0.086x1mm3) (11.75 T). In a sub set of 6 mice, 5 to 7 cine CMR were acquired in LV short axis to cover the whole LV with a lower resolution (0.172?×?0.172x1mm3). We used semi-automatic software to quantify the compacted mass (Mc), the trabeculated mass (Mt) and the percentage of trabeculation (Mt/Mc) on all cine acquisitions. After CMR all hearts were sliced along the short axis and stained with eosin, and histological LV contouring was performed manually, blinded from the CMR results, and Mt, Mc and Mt/Mc were quantified. Intra and interobserver reproducibility was evaluated by computing the intra class correlation coefficient (ICC).ResultsWhole heart acquisition showed no statistical significant difference between trabeculation measured at the basal, midventricular and apical parts of the LV. On the mid-LV cine CMR slice, the median Mt was 0.92 mg (range 0.07–2.56 mg), Mc was 12.24 mg (9.58–17.51 mg), Mt/Mc was 6.74% (0.66–17.33%). There was a strong correlation between CMR and the histology for Mt, Mc and Mt/ Mc with respectively: r2?=?0.94 (p?0.001), r2?=?0.91 (p?0.001), r2?=?0.83 (p?0.001). Intra- and interobserver reproducibility was 0.97 and 0.8 for Mt; 0.98 and 0.97 for Mc; 0.96 and 0.72 for Mt/Mc, respectively and significantly more trabeculation was observed in the Mc Mutant mice than the controls.ConclusionThe proposed semi-automatic quantification software is accurate in comparison to the histology and reproducible in evaluating Mc, Mt and Mt/ Mc on cine CMR. |
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