| Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush |
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| 引用本文： | Yanjuan Sheng1,Yu Zhu2,Lezheng Wu11Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China2Department of Ophthalmology,Zhengzhou University,Zhengzhou 450052,China. Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush[J]. 眼科学报, 2004, 20(3): 181-186 |
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| 作者姓名： | Yanjuan Sheng1 Yu Zhu2 Lezheng Wu11Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China2Department of Ophthalmology Zhengzhou University Zhengzhou 450052 China |
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| 作者单位： | Yanjuan Sheng1，Yu Zhu2，Lezheng Wu11Zhongshan Ophthalmic Center，Sun Yat-sen University，Guangzhou 510060，China2Department of Ophthalmology，Zhengzhou University，Zhengzhou 450052，China |
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| 摘 要： | Traumaticopticneuropathyisanuncommonbutoftendevastatingcauseofpermanentvisuallossafterbluntorpenetratinginjury.Opticnerveiscomposedoftheaxonsofretinalganglioncells(RGC).Opticnerveaxotomycausedrapiddegenerationoftheaxonsandmorethan90%oftheRGCdiedbyapoptosiswithin2weeks[1].AttemptsweremadetopreservetheinjuriedRGCbychangingtheenvironmentorbyupregulatingintrinsicgrowthfactorssurroundingtheRGC[2].Apoptosisisaregulatedprocessunderthecontrolofproteins.TheBcl鄄2genefamilyisoneofthemostimportanta…
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| 关 键 词： | 影响作用 高剂量 甲基强的松龙 老鼠 视网膜中心细胞 细胞凋亡 眼神经 |
Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush |
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| Yanjuan Sheng,YU Zhu,Lezheng Wu. Effect of High Dosage of Methylprednisolone on Rat Retinal Ganglion Cell Apoptosis after Optic Nerve Crush[J]. Eye science, 2004, 20(3): 181-186 |
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| Authors: | Yanjuan Sheng YU Zhu Lezheng Wu |
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| Affiliation: | 1. Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
2. Department of Ophthalmology, Zhengzhou University, Zhengzhou 450052, China |
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| Abstract: | Purpose: To investigate the effect of high dose methylprednisolone (MP) on retinal ganglion cells (RGC) apoptosis.Methods: One hundred and twenty six Wistar rats were divided into normal control, crush control and MP treatment groups. High dose MP and 0.9% sodium chloride were injected intravenously at 1 h after injury in the treatment group and crush control group, respectively. Apoptotic cells, Bcl-2 and Bax positive cells were located and calculated by terminal-deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemical method and retinal whole-mount combined with section technique at day 4, 7, 14 after crush, respectively.Results: The TUNEL-labeling cells, Bcl-2 and Bax positive cells were detected in the ganglion cell layer of retina. Few apoptotic cells, a few of Bcl-2 and Bax positive cells were observed in normal control groups. The numbers of positive cells increased gradually after crush. The number of apoptotic cells and Bax positive cells in treatment groups was less than that in crush group significantly (P < 0.05), while the number of Bcl-2 positive cells in treatment group was more than that in crush group at day 4, 7 and 14 after crush significantly (P < 0.05) Conclusions: These results suggest that high dose MP can inhibit the apoptosis of RGC after optic nerve crush probably through up-regulation of Bcl-2 expression and down-regulation of Bax expression. |
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| Keywords: | methylprednisolone optic nerve injury apoptosis |
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