一步免疫印迹法同时检测抗RA33抗体和抗ENA抗体及其对RA患人的诊断价值

目的 提高抗RA33抗体诊断类风湿性关节炎（RA）的特异性。方法 建立一步免疫印迹法，可同时检测抗RA33抗体和抗ENA抗体。并对266例不同风湿性疾病患者血清和24例正常人血清进行了检测。结果 抗RA33抗体阳性率：RA38％（38/100），SLE35．8％（24/67），MCTD36．4％（8／22），SS8．3％（3/36）。抗RA33抗体阳性血清中抗ENA抗体的检出率：95．8％（23／24）SLE有抗Sm／ulRNP抗体，100％（8／8）MCTD有抗ulRNP抗体，100％（3／3）SS有抗SSA/SSB抗体，RA血清抗ENA抗体均为阴性。结论 本文建立的一步免疫印迹法可有效地鉴别抗RA33抗体阳性的RA和SLE、MCTD等病人。

The use of one-step immunoblotting to detect both anti-RA33 and anti-ENA antibodies, and its diagnostic value for RA patients

Objective To improve the diagnostic specificity of anti-RA33 antibody for rheumatoid arthritis(RA).Methods Establishment of one-step immunoblotting technique to detect both anti-RA33 and anti-ENA antibodies simultaneously,and 266 sera from different rheumatic patients were detected.Results The positive rate of anti-RA33 antibody was 38.0%(38/100) in RA,35.8%(24/67) in SLE,36.4%(8/22) in MCTD,8.3%(3/36) in SS respectively.Among these anti-RA33 positive sera,anti-Sm/u1RNP bands were determinated in 95.8%(23/24) SLE,100%(8/8) MCTD respectively,and anti-SSA/SSB bands were determinated in 100%(3/3) SS sera.In contrast,none of the anti-ENA bands above were found in RA sera.Conclusion The one-step immunblotting in this study is extremely useful to serologically differentiate anti-RA33 positive RA from anti-RA33 positive SLE and MCTD patients.