高脂饮食诱发胰岛素抵抗大鼠模型的建立与评价

目的建立饮食性胰岛素抵抗大鼠模型及评价方法。方法高脂饲料喂养不同年龄和体质量Wistar大鼠,4周后检测血糖、血脂、游离脂肪酸和胰岛素水平并行高胰岛素-正葡萄糖钳夹实验。结果青年高脂组较青年对照组、老年对照组较老年高脂组相比,高脂喂养大鼠体质量虽有所增加,但差异无统计学意义（P均〉0.05）。青年高脂组与青年对照组、老年高脂组与老年对照组、老年对照组与青年对照组及老年高脂组与青年高脂组相比,血清总胆固醇、血清甘油三酯及空腹胰岛素升高,空腹血糖升高,葡萄糖输注率下降,有显著性差异（P〈0.05或0.01）。结论高脂饲料喂养青年和老年大鼠,均可成功建立胰岛素抵抗大鼠模型,钳夹实验用于胰岛素抵抗大鼠模型的评价更准确、可靠。年龄和体质量因素与胰岛素抵抗有关。

Preparation and evaluation of insulin resistance model in rats with high - fat diet

Objective It is to establish and evaluate the insulin resistance model in rats with high-fat diet. Methods Male Wistar rats aged 4 -5 months were divided into young control （YC） group （n = 10） and young high -fat diet .（YHF） group （ n = 10 ） , Male Wistar rats aged 22 - 24 months were divided into old control （ OC ） group （ n = 10 ） and old high-fat diet （OHF） group （ n = 10）. Four weeks later, Body Weight was observed and the serum was separated for the detection of lipid, Glucose, FFA, insulin. The hyperinsulinaemie-euglycaemic clamp was performed to confirm that the insulin resistant rat model was duplicated successfully. Results After four - week high fat diet, Body weight was higher in YHF group than that in YC group, but was not different between the two groups （P 〉 0.05）. That was higher in OHF group than that in OC group, but was not different between two groups too （ P 〉 0.05 ）. Compared with YC group serum total cholesterol, triacylglycerol, fasting insulins and FFA increased in YHF group （P 〈 0.05 or 0.01 ） ; Compared with OC group serum total cholesterol, triacylglycerol, fasting insulins and FFA increased in OHF group （P 〈0.05 or 0.01 ） ; Compared with YC group serum total cholesterol, triacylglyeerol, fasting insulins and FFA increased in OC group （P 〈 0.05 or 0. 01 ） ; Compared with YHF group serum total cholesterol, triaeylglyeerol, fasting insulins and FFA increased in OHF group （P 〈 0.05 or 0. 01 ）. compared with the YC group fasting blood glucose increased and glucose infusion rate decreased in YHF group （P 〈0.05 or 0.01 ） ; Compared with OC group fasting blood glucose increased and glucose infusion rate decreased in OHF group （P 〈 0.05 or 0.01 ） ; Compared with YC group fasting blood glucose increased and glucose infusion rate decreased in OC group （P 〈 0.05 ） ; Compared with YHF group fasting blood glucose increased and glucose infusion rate decreased in OHF group （P 〈 0.05 or 0.01 ）. Conclusion The insulin resistant model can be duplicated successfully in young and old rats with high-fat diet; the hyperinsulinaemic-euglycaemic clamp technique is accurate and reliable in evaluating insulin resistance in rats. Age and weight are related to the induction of insulin resistance.