千金文武汤对胰岛素抵抗模型大鼠瘦素mRNA表达水平的影响

目的]观察千金文武汤对胰岛素抵抗模型大鼠瘦素表达水平的影响.方法]用高脂饲料喂养建立胰岛素抵抗大鼠模型.给予千金文武汤干预治疗,以罗格列酮作为阳性对照组,采用高胰岛素-正常血糖钳夹实验检测大鼠葡萄糖输注率(GIR).取材附睾旁脂肪组织进行瘦素PCR,以观察千金文武汤对胰岛素抵抗大鼠模型的敏感性和脂代谢的影响.结果]经钳夹鉴定胰岛素抵抗模型建立,千金文武汤组与罗格列酮组的GIR较模型对照组显著增高(P<0.05,P<0.01),瘦素PCR扩增后积分光密度表达、mRNA表达水平间差异均有统计学意义(P<0.05,P<0.01).结论]千金文武汤提高胰岛素抵抗大鼠模型的胰岛素敏感性,改善瘦素水平的高表达.与罗格列酮组相比较在脂代谢和瘦素调节上存在差异,与千金文武汤的作用靶点在调节脂代谢和脂肪因子有关联.

Effects of the Qianjin Wenwu decoction on expression of leptin mRNA in insulin resistance rats model

OBJECTIVE To study the effects of Qianjin Wenwu decoction on expression of leptin mRNA in insulin resistance（IR） rats model.METHODS The IR rats model established with feeding high fat diet and categorized equally as the positive control group（IR＋rosiglitazone） and Qianjin Wenwu decoction treated groups（IR＋Qianjin Wenwu decoction）.Glucose infusion rate（GIR） was determined by high insulin-blood glucose clamp experiments.Expression of leptin in epididymal adipose tissue was analyzed by using PCR to observe the effects of leptin for sensitivity and lipid metabolism in IR rats model.RESULTS The GIR was significantly increased in rosiglitazone and Qianjin Wenwu decoction treated groups than in IR group（P0.05,P0.01）,and expression of volume integral optical density and leptin mRNA were markedly increased in two groups（P0.05,P0.01）.CONCLUSION The Qianjin Wenwu decoction enhances the insulin sensitivity of IR rats model and improves high expression of leptin,and its effect target relates to regulating lipid metabolism and fat factor.