固相致敏红细胞吸附技术快速检测肝硬化患者血清中TIMP-1

目的 建立一种新的固相致敏红细胞吸附技术(SPASE)用于快速检测肝硬化患者血清中TIMP-1。方法 用TIMP-1单克隆抗体包被微量血凝板,加热冲洗后加入待检血清标本,最后加入单克隆抗体致敏红细胞。应用致敏红细胞作为指示系统,观察红细胞吸附状况来判定结果。结果 SPASE法全过程仅需要2.5～3小时,408例肝病患者血清检测TIMP-1结果为急性肝炎组检出阳性率16.41％;慢性肝炎组阳性率为33.31％;肝硬化组阳性率为73.58％。结论 血清中TIMP-1的变化可作为肝纤维化较为有用的诊断指标。SPASE法具有较高的灵敏性、特异性和快速性,试剂用量少,且不需复杂的仪器设备,加之实验程序简单,更适于医院及基层医疗单位应用。

SPASE FOR RAPID DETECTION OF SERUM TIMP-1 IN PATIENTS WITH LIVER CIRRHOSIS

Objective Solid-phase absorption to sensitized erythrocytes(SPASE)test is a new technique of solid-phase aggregation. Using SPASE for rapid detction of TIMP-1 in serum in patients with liver cirrhosis. Metods Using antisensitized erythrocytes as indicator to replace enzyme or isotope,the result is judged by observng the agglutination of erythrocytes. A microtitre plate is coated by anti-TIMP-1 McAb. After heating and washing, the serum sample is added to the microtitre plate. The McAb sensitized erythrocytes in put into the microtitre plate lastly. Results The results showed that the whole detection process by SPASE required only 2. 5-3h,it is a specific method for detection of TIMP-1 in scrum and it's very convenient. In addition,it requires only a small amount of reagent,no special apparatus are needed. In 408 serum samples of all, 16. 41 % higher in the group of acute hepatitis,33. 91%higher in the chronic hepatitis ,and 73. 58% higher in the liver cirrhosis group. Conclusion The serum TIMP-1 level is very useful in the assessment of liver fibrosis in chronic liver disease. SPASE is an ideal technique for rapid detection of TIMP-1 from the serum,particularly for rural medical units.