Characterization of secretin release in response to food and intraduodenal administration of fat and hydrochloric acid |
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| Authors: | Dr Edwin J Draviam PhD Dr Guillermo Gomez MD Dr Tsukuru Hashimoto MD Dr Tadashi Miyashita MD Freddie L C Hill BS Tatsuo Uchida MS Pomila Singh PhD George H Greeley Jr PhD James C Thompson MD |
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| Institution: | (1) Department of Surgery and Office of Academic Computing and Biostatistics, The University of Texas Medical Branch, 77550 Galveston, Texas;(2) Present address: Department of Gastroenterology, Pontificia Universidad Catolica de Chile, Santiago, Chile;(3) Present address: First Department of Surgery, Osaka University Medical School, Osaka, Japan;(4) Present address: First Department of Surgery, Kyoto University Medical School, Kyoto, Japan |
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| Abstract: | The development and validation of a radioimmunoassay that detects release of secretin in plasma in response to low doses of secretagogues intraduodenal HCl (0.033 meq/min); intraduodenal sodium oleate (0.04 mmol/min)] or an oral mixed meal in conscious dogs is described. Plasma secretin levels increased significantly (P<0.05) in response to an oral mixed meal in conscious dogs from a basal level of 4.0 to a peak level of 12.3 pg/ml at 15 min. Infusion of graded doses of HCl (2, 4, 8, 16, meq/hr for 30 min) intraduodenally in six dogs resulted in significant elevation of plasma secretin levels in a dose-dependent manner. The pancreatic bicarbonate and volume outputs correlated with the dosage of HCl administered and with the elevations in plasma secretin concentrations. Intraduodenal infusion of increasing doses of sodium oleate (2.4, 4.8, 9.6, and 19.2 mmol in 15-min periods) resulted in a significant (P<0.05) elevation of plasma levels of secretin.Supported by grants from the National Institutes of Health (5R37 DK 15241, PO1 DK 35608). |
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| Keywords: | gastrointestinal hormone radioimmunoassay secretagogues duodenal acidification pancreatic bicarbonate |
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