Molecular characterization and detection of Vicia cryptic virus in different Vicia faba cultivars |
| |
Authors: | R Blawid D Stephan E Maiss |
| |
Institution: | Faculty of Natural Sciences, Institute of Plant Diseases and Plant Protection, Leibniz Universit?t Hannover, Hannover, Germany
|
| |
Abstract: | Summary After extraction of double-stranded (ds) RNAs from Vicia faba, dsRNA1 and dsRNA2 of Vicia cryptic virus (VCV), a member of the genus Alphacryptovirus (family Partitiviridae), were detected in six out of seven different cultivars by agarose gel electrophoresis. In attempts to sequence the complete
VCV genome, the dsRNA1 and dsRNA2 sequences from a total of five different V. faba cultivars were determined. Analysis of these sequences indicated that V. faba cultivars contain almost indistinguishable VCV sequences. The larger dsRNA1 was 2012 bp in length and contained a major open
reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp). The smaller dsRNA2 was 1779 bp in length and
comprised a single ORF on its plus-strand encoding the coat protein (CP). The sequences of the dsRNA1 and dsRNA2 ORFs shared
highest amino acid sequence identities (84 and 56%, respectively) with the corresponding gene products of the alphacryptovirus
white clover cryptic virus 1 (WCCV-1). The 5′-terminal untranslated regions of dsRNA1 and dsRNA2 of VCV were highly conserved
and were strikingly similar to the corresponding regions of WCCV-1. RdRp amino acid sequence alignments revealed conserved
motifs, which correlate with the phylogenetic clustering of the family Partitiviridae. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|