柱前衍生法测定胸腺中组胺的含量

目的建立一种科学、合理的检测胸腺中组胺含量的方法，用于控制胸腺原料的质量。方法采用丹磺酰氯柱前衍生，HPLC-UV法，C18（4．6mm×250mm，5μm）色谱柱，乙腈-水为流动相，流速1．0mL·min-1,检测波长254nm，梯度洗脱。结果采用本方法测定组胺标准品，在1．0～50μg·mL-1的范围内，组胺的浓度与峰面积成线性相关，相关系数为0．9979，加样回收率85％～110％，RSD％〈10％。结论采用该方法测定胸腺中痕量组胺的含量，具有简单、快速、准确、灵敏，重复性好的特点，可以用于胸腺原料的质量控制。

Determination of Histamine in Raw Thymus by Pre-column Derive

Objective To build a scientific and reasonable way to detect the content of histamine in thymus, in order to control the quality of raw thymus ultimately. Methods We used dansyl chloride pre-colunm derive, HPLC-UV method, C18 （4.6mm×250mm, 5μm） chromatographic column acetonitrile-water as a mobile phase, the flow rate 1.0mL·min-1, wavelength 254nm while the gradient elution method was used to detect histamine. Results In the range of 1.0-50μg·mL-1, the histamine concentration is linear correlation to the peak area, with the correlation coef- ficient 0.9979. Sample loading recovery is between 85% and 110%, with RSD%〈10%. Conclusion Using this way to detect the histamine content in thymus is simple, quick, accurate, sensitive, and easy to repeat. This method can be used as quality control of raw.