| 体外培养兔视网膜细胞损伤后的形态变化 |
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| 引用本文： | 蔡莉,惠延年,孟晋宏,杨瑞华. 体外培养兔视网膜细胞损伤后的形态变化[J]. 眼科学报, 2000, 16(2): 127-130 |
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| 作者姓名： | 蔡莉 惠延年 孟晋宏 杨瑞华 |
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| 作者单位： | 第四军医大学西京医院眼科，西安 710032 |
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| 基金项目： | 国家自然科学基金资助,基金号:39900076 |
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| 摘 要： | 目的：观察体外培养的兔视网膜细胞在受到机械损伤后的形态变化。方法：建立乳兔视网膜细胞及与Ｓｃｈｗａｎｎ Ｃｅｌｌ（ＳＣ）共培养模型，采用机械刮损方法对视网膜细胞造成损伤，相差显微镜下连续２４小时观察细胞对损伤的反应。结果：刮损后３０分钟胶质细胞逐渐移向损伤区，分裂增殖并填补裸区。神经元突起在损伤后６小时开始再生，其生长方向受到周围环境影响，具有“可塑性”。与ＳＣ共培养时，损伤后３小时即可发现再生突
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| 关 键 词： | 细胞培养 视网膜 神经元 胶质细胞 损伤 |
Morphological Changes of Cultured Rabbit Retinal Cells after Injury |
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| Li Cai,Yannian Hui,Jinhong Meng,Ruihua YangDept. of Ophthalmology,Xijing Hospital,The th Military Medical University,XiAn ,China. Morphological Changes of Cultured Rabbit Retinal Cells after Injury[J]. Eye science, 2000, 16(2): 127-130 |
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| Authors: | Li Cai Yannian Hui Jinhong Meng Ruihua YangDept. of Ophthalmology Xijing Hospital The th Military Medical University XiAn China |
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| Affiliation: | Dept. of Ophthalmology, Xijing Hospital, 4th Military Medical University, XiAn 710032, China. |
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| Abstract: | Purpose: To investigate the morphological changes of cultured rabbit retinal cells after injury.Methods: Mechanical scratch was done in nenatol rabbit retinal cell culture and in Schwann Cell(SC)/retinal cell colculture. The morphological changes of different sorts of cells after the injury were observed under the phase contrast microscope for 24h. Results: About 30min after injury, many glial cells moved to the blank region and proliferated to form gliosis. Axons of retinal neurons began to regenerate 6h post injury. The direction of regrowth was influenced by the environment. In the coculture, regeneration of axons was discerned only 3h after injury.Conclusion: Axons of retinal neurons had strong capability of regeneration; Gliosis was not the absolute obstacle of regeneration; SC played a role in promoting the axon regeneration. Eye Science 2000; 16: 127 - 130. |
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| Keywords: | cell culture retina/neurons Glia cells injury |
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