| 嗅痛灵嗅剂的质量标准研究 |
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| 引用本文： | 陈智伟,黄庆宝,付文洋,徐汉明.嗅痛灵嗅剂的质量标准研究[J].内蒙古中医药,2014,33(25):84-85. |
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| 作者姓名： | 陈智伟 黄庆宝 付文洋 徐汉明 |
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| 作者单位： | 广东深圳市中医院,518033 |
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| 摘 要： | 目的:建立HPLC测定嗅痛灵嗅剂中细辛木脂素含量的方法。方法:采用薄层色谱法鉴别嗅痛灵嗅剂中木香、细辛;采用HPLC对制剂中细辛木脂素进行含量测定,色谱柱:GL Inertsil ODS-SP色谱柱(5μm,150mm×4.6mm);流动相:乙腈:水溶液梯度洗脱;流速:1ml/min;检测波长:287nm;柱温:40℃。结果:木香、细辛的薄层鉴别斑点清晰、专属性强;细辛木脂素在15.16744~75.8372μg呈良好线性关系。(r=9999,n=5),平均回收率为97.88%,RSD%为1.18%(n=9)。结论:本研究建立的质量标准方法操作简单、专属性强、重现性好、结果准确可靠。
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| 关 键 词： | 嗅痛灵嗅剂 细辛木脂素 高效液相色谱法 |
Determination of Herba asari lignin in Xiutongling Snuff by HPLC |
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| Chen zhiwei,Huang qingbao,Fu wenyang,Xu hanming.Determination of Herba asari lignin in Xiutongling Snuff by HPLC[J].Nei Mongol Journal of Traditional Chinese Medicine,2014,33(25):84-85. |
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| Authors: | Chen zhiwei Huang qingbao Fu wenyang Xu hanming |
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| Institution: | (The Hospital of Traditional Chinese Medicine in Shenzhen City, Shenzhen 518033, China ) |
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| Abstract: | Objective To estai）lish a method for the determination of HPLC concentrations of lignan olfactory pain spirit snuff. Methods： TLC method of olfactory pain spirit snuff in woody, asarum; using HPLC to preparation of Herba asari lignin content determination, chro- matographic column： GL Inertsil ODS-SP column （5μm, 150mm× 4.6mm）; mobile phase： aeetonitrile： water solution gradient elution; flow rate： Iml/ rain; the detection wavelength： 287nm; column temperature： 40 ℃. Result： The TLC spots Radix Aueldandiae, asarum clear, strong specificity; Herba asari lignin in 15.16744 - 75.8372μg showed a good linear relationship. （r=9999, n=5）, the average re- covery was 97.88%, RSD% was 1.18% （n=9）. Conclusion： The quality standard of the method has the advantages of simple operation, strong specificity, good reproducibility, and the result is accurate and reliable. |
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| Keywords: | Xiutongling Snuff Herba asari lignin HPLC |
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