| HPLC测定益肾化浊颗粒中丹酚酸B的含量 |
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| 引用本文： | 孟蕾,曹杰,费超,张海鸣,杜树山. HPLC测定益肾化浊颗粒中丹酚酸B的含量[J]. 中国中医药信息杂志, 2011, 18(10): 53-54. DOI: 10.3969/j.issn.1005-5304.2011.10.020 |
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| 作者姓名： | 孟蕾 曹杰 费超 张海鸣 杜树山 |
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| 作者单位： | 1. 北京师范大学校医院,北京,100875
2. 中药资源保护与利用北京市重点实验室,北京,100875 |
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| 基金项目： | 北京市新医药学科群重点支持项目 |
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| 摘 要： | 目的建立益肾化浊颗粒中丹酚酸B含量的高效液相色谱测定方法。方法采用Waters AtlantisTM d C18色谱柱（3.9 mm×150 mm,5μm）,以乙腈-甲醇-2%醋酸水溶液（18∶1∶81）为流动相,流速为1.0 mL/min,检测波长为286 nm,柱温30℃。结果丹酚酸B线性范围为0.12～1.57μg（r=0.9999）,平均加样回收率为98.41%,RSD=0.69%（n=6）。结论本方法快速、简便、准确、重复性较好,结果可靠,可用于控制益肾化浊颗粒的质量。
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| 关 键 词： | 益肾化浊颗粒 丹酚酸B 高效液相色谱法 |
Determination of Salvianolic Acid B in Yishenhuazhuo Granules by HPLC |
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| MENG Lei,CAO Jie,FEI Chao,ZHANG Hai-ming,DU Shu-shan. Determination of Salvianolic Acid B in Yishenhuazhuo Granules by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2011, 18(10): 53-54. DOI: 10.3969/j.issn.1005-5304.2011.10.020 |
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| Authors: | MENG Lei CAO Jie FEI Chao ZHANG Hai-ming DU Shu-shan |
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| Affiliation: | MENG Lei1,CAO Jie2,FEI Chao2,ZHANG Hai-ming2,DU Shu-shan2(1.School Hospital of Beijing Normal University,Beijing 100875,China,2.Protection and Utilization of Traditional Chinese Medicine of Beijing Area Major Laboratory,China) |
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| Abstract: | Objective To estalish an HPLC method for the determination of salvianolic acid B in Yishenhuazhuo Granules.Methods Waters AtlantisTM d C18（3.9 mm×150 mm,5 μm） column in an oven at 30 ℃ was adopted,with a mobile phase consisting of acetonitrile-methanol-2% acetic acid solution（18∶1∶81） and a UV detector at 286 nm,the flow rate was 1.0 mL/min.Results The linear ranges of salvianolic acid B was 0.12-1.57 μg（r=0.999 9）.The average recovery was 98.41%（RSD=0.69%,n=6）.Conclusion The method was simple and accurate,and can be used for the quality control of Yishenhuazhuo Granules. |
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| Keywords: | Yishenhuazhuo Granules salvianolic acid B HPLC |
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