| 大豆甙元对子宫内膜癌细胞增殖的影响及其受体作用机制研究 |
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| 引用本文： | 刘小丽,薛晓鸥,赵丽云,牛建昭.大豆甙元对子宫内膜癌细胞增殖的影响及其受体作用机制研究[J].中国中医药信息杂志,2011,18(10):45-48. |
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| 作者姓名： | 刘小丽 薛晓鸥 赵丽云 牛建昭 |
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| 作者单位： | 1. 北京中医药大学东直门医院妇科,北京,100700
2. 北京中医药大学基础医学院,北京,100029 |
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| 基金项目： | “十一五”国家科技支撑计划，首都医学科技发展基金 |
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| 摘 要： | 目的观察大豆甙元对子宫内膜癌细胞增殖及其雌激素受体（ER）表达的影响。方法体外培养子宫内膜癌HEC-1B细胞,采用MTT法检测不同浓度大豆甙元对子宫内膜癌细胞增殖的影响,同时检测大豆甙元对启动子（ERE）调控的ERα和ERβ的荧光素酶报告基因表达的影响。结果大豆甙元对HEC-1B细胞体外增殖有明显抑制作用,其抑制作用在一定范围内呈剂量及时间依赖性。大豆甙元在低浓度时已可显著诱导报告基因luc的表达,在80×10-6 mol/L时达到最高值;在高浓度时,其诱导作用逐渐下降;大豆甙元通过ERβ介导的报告基因表达水平的升高程度强于ERα。结论大豆甙元可抑制子宫内膜癌细胞的增殖,这种抑制作用可通过调节子宫内膜癌细胞ERα和ERβ的表达、调整ERα/ERβ比例而实现。
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| 关 键 词： | 大豆甙元 子宫内膜癌 细胞增殖 雌激素受体 报告基因 |
Effect of Daidzein on Proliferation of Endometrial Carcinoma and Its Mechanism |
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| LIU Xiao-li,XUE Xiao-ou,ZHAO Li-yun,NIU Jian-zhao.Effect of Daidzein on Proliferation of Endometrial Carcinoma and Its Mechanism[J].Chinese Journal of Information on Traditional Chinese Medicine,2011,18(10):45-48. |
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| Authors: | LIU Xiao-li XUE Xiao-ou ZHAO Li-yun NIU Jian-zhao |
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| Institution: | LIU Xiao-li1,XUE Xiao-ou1,ZHAO Li-yun2,NIU Jian-zhao2(1.Department of Gynecology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China,2.Institute of Basic Medical Science,Beijing 100029,China) |
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| Abstract: | Objective To study the effect of daidzein on the proliferation of endometrial carcinoma and expression of estrogen receptor(ER).Methods Endometrial carcinoma cell HEC-1B were cultured in vitro.The influences of daidzein in different doses on the proliferation of HEC-1B cell were detected by using MTT method,and the estrogenic effects and the mechanism of daidzein were evaluated through the luciferase reportor gene assay.Results Daidzein inhibited significantly the proliferation of HEC-1B cells in vitro,and ... |
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| Keywords: | daidzein endometrial caicinoma proliferation estrogen receptor reportor gene |
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