|
|||||
|
|
| 清开灵注射液对血脑屏障模型通透性的影响 | |
| 引用本文： | 朱海燕,迟蕾,徐冰,高永红,娄晋宁.清开灵注射液对血脑屏障模型通透性的影响[J].中国中医药信息杂志,2011,0(9):35-37. |
| 作者姓名： | 朱海燕 迟蕾 徐冰 高永红 娄晋宁 |
| 作者单位： | 1. 北京师范大学资源学院,北京100875;中药资源保护与利用北京市重点实验室,北京100875 2. 北京中医药大学东方医院,北京,100078 3. 北京中医药大学东直门医院中医内科学教育部重点实验室,北京,100700 4. 中日友好医院临床研究所,北京,100029 |
| 摘 要： | 目的通过观察清开灵注射液对血脑屏障（BBB）模型通透性的影响,探究其抗脑缺血再灌注损伤作用的可能机制及靶点。方法采用细胞插入器培养单层脑微血管内皮细胞,建立体外BBB模型,三气培养箱中1%O2模拟缺氧培养24 h,分为正常组、缺氧组、清开灵小剂量组和大剂量组,通过对跨内皮细胞电阻（TEER）和辣根过氧化物酶（HRP）透过率的测定,观察清开灵注射液对缺氧损伤BBB通透性的影响。结果清开灵注射液可改善缺氧对BBB造成的损伤,提高TEER,降低跨内皮细胞通透性,并且有剂量依赖的趋势,清开灵注射液大剂量组与缺氧组比较,差异有统计学意义（P〈0.01）,清开灵注射液小剂量组改善作用虽不如大剂量组显著,但亦能减少HRP透过BBB（P〈0.05）。结论清开灵注射液能有效改善缺氧损伤造成的BBB功能受损。 |
| 关 键 词： | 清开灵注射液 血脑屏障 通透性 缺氧 |
Effect of Qingkailing Injection on Blood Brain Barrier Permeability in Hypoxia Condition |
|
| ZHU Hai-yan,CHI Lei,XU Bing,GAO Yong-hong,LOU Jin-ning.Effect of Qingkailing Injection on Blood Brain Barrier Permeability in Hypoxia Condition[J].Chinese Journal of Information on Traditional Chinese Medicine,2011,0(9):35-37. | |
| Authors: | ZHU Hai-yan CHI Lei XU Bing GAO Yong-hong LOU Jin-ning |
| Institution: | ZHU Hai-yan1,2,CHI Lei3,XU Bing4,GAO Yong-hong4,LOU Jin-ning5(1.College of Resource Science & Technology,Beijing Normal University,Beijing 100875,China,2.Beijing Key Laboratory of TCM Protection and Utilization,3.Dongfang Hospital Affiliated to Beijing University of Chinese Medicine,Beijng 100078,4.The Ministry of Education Key Laboratory of Internal Medicine Department of TCM,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,5.Clinical Reaear... |
| Abstract: | Objective By observing the effect of Qingkailing injection on blood brain barrier(BBB) permeability in vitro,explore the possible mechanism and its target anti-cerebral ischemia-reperfusion injury.Methods The BBB model in vitro was established by culturing cerebral microvasculature endothelial cells in Cell Culture Inserts,anoxia in 1% O2 three gas incubator for 24 hours,divided into normal group,hypoxia group,Qingkailing small dose group and high dose group.The effect of Qingkailing injection on permeabili... |
| Keywords: | Qingkailing injection blood brain barrier permeability hypoxia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |