| 大黄素通过激活PPARδ促进胰高血糖素样肽1分泌的研究 |
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| 引用本文： | 周丽嫦,徐艳燕,陈伟标,曾婉君,王叶茗,欧阳勇.大黄素通过激活PPARδ促进胰高血糖素样肽1分泌的研究[J].中国中医药现代远程教育,2011,9(21):142-144. |
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| 作者姓名： | 周丽嫦 徐艳燕 陈伟标 曾婉君 王叶茗 欧阳勇 |
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| 作者单位： | 广东省广州市中西医结合医院药剂科,广州,510800 |
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| 摘 要： | 目的探讨中药活性成分大黄素对胰高血糖素样肽1(GLP-1)的分泌的影响及机制。方法①以高糖培养基(DMEM)培养小鼠肠道肿瘤内分泌细胞株(STC-1)进行体外的实验研究,以ELISA法检测GLP-1含量,探索大黄素体外对STC-1分泌GLP-1的影响。②利用C57BL/6J小鼠进行体内实验研究,分析小鼠给予大黄素灌胃对口服葡萄糖负荷的小鼠分泌GLP-1及胰岛素的影响。结果大黄素可剂量依赖性地促进STC-1细胞株分泌GLP-1,PPARδ特异性阻断剂GSK0660可阻断大黄素的作用;大黄素灌胃可提高口服负荷葡萄糖小鼠血浆GLP-1的水平,但同样可被GSK0660所阻断。结论体外和体内实验证实,大黄素可促进GLP-1分泌,该作用可能与激活代谢性核受体PPARδ亚型有关。
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| 关 键 词： | 大黄素 GLP-1 STC-1 PPAR δ |
Emodin Increase GLP-1 Secretion through the Activation of PPAR δ |
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| Zhou Lichang,Xu Yanyan,Chen Weibiao,Zeng Wanjun,Wang Yeming,Ou Yangyong.Emodin Increase GLP-1 Secretion through the Activation of PPAR δ[J].Chinese Medicine Modern Distance Education of China,2011,9(21):142-144. |
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| Authors: | Zhou Lichang Xu Yanyan Chen Weibiao Zeng Wanjun Wang Yeming Ou Yangyong |
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| Institution: | Zhou Lichang,Xu Yanyan,Chen Weibiao,Zeng Wanjun,Wang Yeming,Ou Yangyong Guangzhou Hospital of Integrated Chinese and Western medicine,GuangDong province China 510800 |
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| Abstract: | Objective To investigate whether the emodin can increase the GLP-1 secretion in vitro and vivo.Mehtods Culture the STC-1 cell line with high glucose DMEM to study the effect of emidin to the secretion of GLP-1,use the method of ELISA to detect the concentration of GLP-1.In vivo study,we use the C57BL/6J mice to study whether the oral administration of edomin can increase the GLP-1 and insulin secretion in vivo after the challenge of glucose solution.Results Emodin increased the secretion of GLP-1 in a dose ... |
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| Keywords: | Emodin GLP-1 STC-1 PPAR δ |
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