黑水缬草药材质量标准研究

目的建立黑水缬草中缬草素的薄层色谱鉴别方法和缬草烯酸的含量测定方法。方法采用薄层色谱法（TLC）对黑水缬草进行定性鉴别。采用高效液相色谱法（HPLC）测定缬草烯酸含量,采用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）;流动相：甲醇-0.5%磷酸梯度洗脱;检测波长：268 nm;柱温：35℃;流速：1 mL/min。结果各批黑水缬草药材TLC中均能检出缬草素;缬草烯酸HPLC色谱峰与其他色谱峰分离良好,进样量在0.074 7～1.195 2μg范围内呈良好线性关系（r=0.9999）;缬草烯酸的加样回收率为99.33%,RSD=1.36%。结论本法简便快捷,结果可靠,为控制黑水缬草药材的质量提供了依据。

Study on Quality Standard of Valeriana amurensis

Objective To establish the TLC identification and quantitative determination method of Valeriana amurensis. Methods TLC was adopted to identify and HPLC was used to determine valerenic acid in Valeriana amurensis. The chromatographic condition was as follows： Diamonsil C18 （4.6 mm× 250 mm, 5μm） column, methanol-0.5%H3PO4 water as mobile phase for gradient elution, detection wavelength at 268 nm, column temperature at 35 ℃, flow rate at 1 mL/min. Results The valepotriate was identified by TLC and the valerenic acid was determined by HPLC. The liner range of valerenic acid was 0.074 7-1.195 2μg, r=0.999 9. The average recovery was 99.33%, and RSD was 1.36%. Conclusion The methods are convenient, accurate and reproducible, and can be used to control the quality of Valeriana amurensis.