Isolation and characterization of myosin from the adrenal medulla |
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Authors: | Trifaró J M Ulpian C |
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Affiliation: | Department of Pharmacology and Therapeutics, McGill University, Montreal, H3G 1Y6, Quebec, Canada. |
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Abstract: | A protein with adenosine triphosphatase activity was isolated from bovine adrenal medulla and subsequently purified by ammonium sulfate precipitation and agarose gel filtration using a discontinuous two-buffer system. Characterization of this protein by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate, by assay of the activity of Ca2+, K(+)-EDTA and Mg2+ dependent adenosine triphosphatases by amino acid analysis and by electron microscopy has shown that the adrenal medullary myosin closely resembles those myosins isolated from muscle and other non-muscle cells. The possible roles of myosin in the adrenal medulla are discussed. |
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Keywords: | ATP adenosine 5'-triphosphate ATPase adenosine triphosphatase EDTA ethylenediamine tetraacetate TES N-tris(hydroxymethyl)methyl-2-aminoethanesulfonate partition coefficient |
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