Ad-HGF修饰MSCs异体移植对烧伤创面愈合的作用

目的：探讨携带人肝细胞生长因子基因的重组腺病毒（Ad—HGF）修饰的骨髓间充质干细胞（MSCs）局部异体移植对烧伤创面愈合的作用。方法：密度梯度离心法体外分离培养雄性Wistar大鼠MSCs，并以感染复数（MOI=100）体外转染Ad—HGF。30只雌性Wistar大鼠随机分为：MSCs治疗组（A组）。Ad—HGF修饰MSCs治疗组（B组），Ad—GFP修饰MSCs治疗组（C组），Ad—HGF治疗组（D组）。空白对照组（E组）。复制大鼠深Ⅱ度烧伤模型，将细胞悬液、病毒悬液及生理盐水于创面下多点注射。伤后3、5、7、14、21d观察创面收缩率并采集创面标本，行HE染色观察创面愈合情况。结果：伤后7、14dB组与A组、C组、D组、E组比较，创口收缩率显著提高（P〈0．05）；HE染色可见B组表皮明显厚于其他各组。并可见钉脚下伸。结论：Ad—HGF修饰MSCs异体移植可显著提高烧伤创面愈合速度和质量，为烧伤创面修复提供了新的策略。

Effects of Ad-HGF modified mesenchymal stem cells on healing of burn wounds after heterogenous grafting

Objective:To explore the effects of bone mesenchymal stem cells(MSCs) with transfection of adenoviral vector expressing human hepatocyte growth factor(Ad-HGF) after heterogenous grafting on healing of burn wounds.Methods: MSCs of male Wistar rats were collected and cultured,and were transfected with Ad-HGF.Thirty animals were subjected to boiling water for 12 seconds to produce a partial thickness skin burn.The rats were randomly divided into 5 groups: MSCs treatment(group A),transplantation of Ad-HGF modified MSCs(group B),transplantation of Ad-GFP modified MSCs(group C),Ad-HGF treatment(group D),and saline control(group E).The suspension of MSCs,suspension of Ad-HGF and saline were injected into the derma of burn wounds.The contraction rate of burn wounds was calculated and histological examination was performed 3,5,7,14 and 21 days after the injury.Results: During the first and second week after burn,the contraction rate of burn wounds in group B was much higher than that in group A, C,D and E(P<0.05).Histological examination showed the thickness of epidermis in group B was significantly deeper than that in the other groups,and the nails of dermis were inserted into derma of burn wounds.Conclusion: Transplantation of MSCs with Ad-HGF transfection has positive effects on the healing of burn wounds,which may provide a new way to burn wound repair.