吡格列酮对鼠角膜碱烧伤后新生血管抑制作用的研究

目的:探讨吡格列酮对大鼠碱烧伤后角膜血管内皮细胞生长因子(VEGF),碱性成纤维细胞生长因子(b-FGF)表达的调节作用,以及对碱烧伤后角膜新生血管生长的抑制作用。方法:建立大鼠角膜碱烧伤模型,结膜下注射吡格列酮,观察烧伤后不同时期的角膜新生血管的数量、长度,应用免疫组化和Western blot方法检测碱烧伤后及烧伤治疗不同时期的VEGF,b-FGF的表达变化情况,并与对照组比较。结果:大鼠角膜碱烧伤后2d角膜新生血管开始生长,7～10d达到高峰,吡格列酮治疗后的角膜新生血管的发生延迟,血管的生长受到抑制,VEGF,b-FGF随时间发生变化,表达较对照组下降。结论:局部应用吡格列酮对由碱烧伤引起的鼠角膜新生血管的发生、发展有明显的抑制作用,其机理可能是通过下调促血管生成因子VEGF,b-FGF的表达而实现的。

Inhibitory effects of pioglitazone on alkaline burn-induced corneal neovascularization in rats

AIM:To investigate the effects of pioglitazone on corneal neovascularization(CNV) induced by alkali burnin rats and on expression of vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(b-FGF) .METHODS:CNV was induced by alkaline burn in rats which were then administered pioglitazone subconjunctivally.Corneal angiogenesis was evaluated and observed with a slit lamp microscope and the VEGF and b-FGF level were determined by immunohistochemistry and Western blot at different intervals.RESULTS:The CNV of the rats was shown two days after alkaline burn,and reached its highest level in 7-10 days.The mean values of the area and length of neovascularization and the VEGF and b-FGF levels in the pioglitazone-treated group were significantly lower than those in the control group at various time.CONCLUSION:Local application of pioglitazone could remarkably inhibit CNV,probably by means of downregulation of VEGF and b-FGF expression.