醋酸氯己定与替硝唑联合药敏试验观察

目的研究醋酸氯己定与替硝唑协同抗菌效果。方法采用棋盘方阵稀释法,对醋酸氯己定与替硝唑联合应用体外抗菌作用进行了观察。结果测定替硝唑和醋酸氯己定对金黄色葡萄球菌、铜绿假单胞菌、产气荚膜梭菌和脆弱拟杆菌的MIC值,替硝唑分别为200、200、1、1μg/ml,醋酸氯己定分别为0.06、4、1、4μg/ml。替硝唑与醋酸氯己定联合应用对上述4种试验菌的MIC值与各自的单方相比较无变化,两药相加无相关作用,但相互也不干扰对方的抗菌作用。结论应用于创伤涂膜剂中的醋酸氯己定和替硝唑两种主要抗菌成分既无协同作用或相加作用,但也不互相干扰。

OBSERVATION ON COMBINED CHEMOSENSITIVITY TEST OF CHLORHEXIDINE ACETATE AND TINIDAZOLE

Objective To study synergistic antibacterial effect of chlorhexidine acetate and tinidazole.Methods Checkerboard square dilution method was used to observe in vitro antibacterial effects of chlorhexidine acetate combined application with tinidazole.Results MIC value of chlorhexidine acetate to Staphylococcus aureus,Pseudomonas aeruginosa,Clostridium perfringens and Bacteroides fragilis were 0.06,4,1,4 μg/ml respectively.MIC value of tinidazole were 200,200,1,1 μg/ml respectively.MIC value of tinidazole and chlorhexidine acetate combined with the application to the four kinds of test bacteria had no change to single use.Conclusions Applied the two main antimicrobial component to the trauma liniment has no synergistic effect or additive effects,but also do not interfere with each other.