乳腺癌与MCF-7乳腺癌细胞株中SP含量的比较

目的鉴定乳腺癌组织、癌旁组织、正常组织与MCF-7细胞系中SP（side population）亚群含量是否有差异．方法制备乳腺癌组织、癌旁组织、正常组织细胞悬液和MCF-7细胞悬液，一组加入Hoechst33342染色，另一组同时加入拮抗剂维拉帕米，通过流式细胞仪测定sP亚群含量．结果SP亚群在癌组织、癌旁组织、正常组织和MCF-7细胞的含量分别为（0．54±0．17）％、（7．23±1．12）％、（8．34±0．96）％和（9．58±1．27）％，且它们之间的SP亚群含量具有差异显著性．结论人乳腺癌组织、癌旁组织、正常组织与MCF-7细胞系中都存在SP亚群且含量不同，能够为肿瘤干细胞的鉴定和提纯提供一定的科学参考价值．

Comparison of SP Cells between Breast Cancer Tissues and MCF-T Cells

Objective To identify whether the percentages of SP cells have differences in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cells. Methods After staining with Hoechst 3 3342 dye and Verapamil, the percentages of SP cells were detected in cell suspension from breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line respectively with the FACS method. Results The percentages of SP cells in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line were （0.54 ＋ 0.17）%, （7.23 ＋ 1.12）%, （8.34 ＋ 0.96）% and （9.58 ＋ 1.27）%, respectively. There were significant differences between them. Conclusions Side population cells express in breast cancer tissues, tumor-adjacent tissues, normal tissues and MCF-7 cancer cell line, and the percentages of SP cells are different. This result can supply the scientific value to identify and purify breast cancer stem cells.