Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations |
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Authors: | Sandrine Chabane Emmanuel Gachet François Képès |
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Institution: | (1) Service de Biochimie et de Génétique Moléculaire, DBCM/DSV, Bat. 142, CEA/Saclay, F-91191 Gif cedex, France Fax: (33) 1 69 08 47 12 e-mail: kepes@jonas.saclay.cea.fr, FR |
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Abstract: | The fungal metabolite Brefeldin A (BFA) disrupts the Golgi apparatus and its incoming protein flux. We developed a genetic
approach to identify yeast proteins involved in the protein transport step that BFA blocks. The BFR2 gene (YDR299W) was thus isolated as a high-copy suppressor of the growth defects induced by BFA in a sensitive strain of
Saccharomyces cerevisiae. Although BFR2 over-expression did not cause a secretory block or slow-down, it partially suppressed the growth defect of four mutants blocked
at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1). The essential BFR2 gene was predicted to encode an extremely hydrophilic product containing two short regions with potential coiled-coils, one
of which corresponds to a cluster of acidic residues.
Received: 10 September / 3 November 1997 |
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Keywords: | Saccharomyces cerevisiae Protein transport Golgi apparatus Brefeldin A |
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