大鼠出血性休克再灌注肾损伤的一种新模型

目的 :探讨一种新的出血性休克再灌注肾损伤 (HS RRI)的大鼠模型。方法 :30只SD大鼠完全随机分为S3 0 、S40 、S50 、S60 、S3 组共 5组 ,其中前 4组模型依据文献建立 ,S3 组是新模型 ,选用失血量、平均动脉压 (MAP)、休克末尿素氮(BUN)及肌酐 (Cr)、再灌注 2 4h肾组织病理损伤等指标。结果 :S3 组复苏成功率 83.33%。各组在复苏 30min时的MAP与其休克前相比差异均显著 (P <0 .0 1)。S3 组和S40 组的失血量、BUN、Cr、肾组织病理损伤积分与其他 4组的差异均显著 (P <0 .0 5 ) ,其中S3 组肾损伤最明显 ,S40 组最轻。结论 :S3 组的大鼠模型 (MAP 4 0mmHg ,休克 3h)是再灌注肾损伤较严重的模型。

The New SD Rat Model of Renal Reperfusion Injury Following Hemorrhagic Shock

Objective: To explore a new rat model of renal reperfusion injury following hemorrhagic shock. Methods: 30 SD rats were randomly divided into 5 groups: S 30 , S 40 , S 50 , S 60 and S 3. Models in group S 30 , S 40 , S 50 , S 60 were made according to the literatures. When making the new models in group S 3, indexes on bleedout, MAP, BUN, Cr at the end of the shock, and renal pathologic features 24 hours after reperfusion were considered. Results: The successful ratio in group S 3 was 83.33%. There was significant change in MAP before the shock and 30 min after reperfusion in all groups (P<0.01). Group S 3 and group S 40 were significantly different from the other groups in bleedout, BUN, Cr and renal pathologic integral (P<0.05), with group S 3 the highest and group S 40 the lowest in renal pathologic integral. Conclusion: The models in group S 3 (MAP=40 mmHg, time of shock=3 h) had the most severe renal reperfusion injury in all groups.