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Synthesis and properties of human β-endorphin-(1–17) and its analogs |
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| Authors: | RAFFAELLA GARZIA DONALD YAMASHIRO R. GLENN HAMMONDS CHOH HAO LI |
| Abstract: | Four analogs of human β-endorphin (βh-EP) were synthesized by the solid-phase method: βh-EP-(1–17) (I), [D-Ala2]-βh-EP-(1–17) (II), [Gln8]-βh-EP-(1–17) (III) and [D-Ala2, Gln8]-βh-EP-(1–17) (IV). Measurement in a radio-receptor binding assay with use of tritiated βh-EP as primary ligand gave relative potencies as follows: Met-enkephalin, 100; I, 33; II, 47; III, 889; IV, 123; βh-endorphin, 2253. |
| Keywords: | γ -endorphin Met-enkephalin opiate receptor-binding assay solid-phase peptide synthesis |