应用微孔噬菌体扩增法检测结核分枝杆菌对利福平的敏感性

目的　应用并评价微孔噬菌体扩增法 (micro wellphagereplicationassay ,MPRA)快速检测临床结核分枝杆菌分离株对利福平的敏感性。方法　将适当浓度的利福平溶液作用于结核分枝杆菌临床分离株 ,一定时间后 ,加入噬菌体悬液。应用MPRA法检测利福平药物的敏感性。结果　应用绝对浓度法对利福平敏感的 4 2株菌株中 38株MPRA法敏感 ;绝对浓度法对利福平耐药的 131株菌株中 12 4株MPRA法耐药。MPRA法与绝对浓度法的符合率为 93 6 % (16 2 / 173) ,与绝对浓度法相比 ,MPRA法的敏感度为 94 7% (12 4 / 131) ,特异度为 90 5 % (38/ 4 2 )。结论　MPRA法不失为一种快速检测结核分枝杆菌对利福平敏感性的较好方法

The Study of the Susceptibility of M.Tuberculosis to Rifampin by Micro-well Phage Replication Assay

Objective To assess susceptibility of to 173 clinical M.tuberculosis isolates to by Micro-well Phage Replication Assay(MPRA).Methods To prepare the isolates and expose them to rifampin . To prepare phage D29 suspension . MPRA array for the susceptibility to rifampin.Results Compared with the absolute concentration method ,there were the same result of 38 isolate in 42 susceptible strains and of 124 isolate in 131 resistant strains. Between result of MPRA assay and absolute concentrstion (method,) concordance was 93.6%(38/42), susceptibility was 94.9%(124/131) and specificity is 90.5%((38/42).)Conclusion MPRA assay is a good and rapid method for drug susceptibility of rifampin.