Tick fauna in non-anthropogenic areas in Mato Grosso do Sul,Brazil, with the presence of the Rickettsia parkeri strain Atlantic rainforest in Amblyomma ovale |
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| Institution: | 1. Bolsista Fundapam/Laboratório de Biologia do Carrapato, Embrapa Gado de Corte, Campo Grande, MS, Brazil;2. Laboratório de Ixodologia/Doutorando no Programa de Pós-Graduação em Imunologia e Parasitologia Aplicadas da Universidade Federal de Uberlândia. Av. Amazonas s/n, Campus Umuarama-Bloco 6T, Uberlândia, MG, 38405-302, Brazil;3. Fundação Oswaldo Cruz, Fiocruz Rondônia, Rua da Beira, No. 7671, Bairro Lagoa, CEP 76812-245 Porto Velho, RO, Brazil;4. Post Doctorate/Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA;5. Universidade Estadual de Mato Grosso do Sul/Rodovia Aquidauana/UEMS - km 12;6. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias INIFAP- Vera Cruz, México;7. Embrapa Gado de Corte, Avenida Radio Maia, 830, Campo Grande, MS CEP 79106-550, Brazil;1. Institute for Hygiene and Applied Immunology; Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria;2. Department of Environmental Health, Center for Public Health, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria;1. Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch 6150, Western Australia, Australia;2. Behavioural Ecology Lab, School of Molecular and Life Sciences, Curtin University, Bentley 6102, Western Australia, Australia;1. Institute of Chemistry, Biological Chemistry Laboratory, Universidade Estadual de Campinas, CEP 13083-970, CP 6152, Campinas, SP, Brazil;2. Biochemistry Department, Institute of Biology, Universidade Estadual de Campinas, Campinas, SP, Brazil;3. Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, RN, Brazil;4. NanoBioss (MCTI), Institute of Chemistry, Universidade Estadual de Campinas, Campinas, SP, Brazil;5. Brazilian Nanotechnology National Laboratory (LNNano-CNPEM), Campinas, SP, Brazil;1. Doctorado en Ciencias, Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales. Caldas, Colombia;2. Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia;3. Grupo de Investigación Parasitología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Carrera 30 No.45-03, 111321, Bogotá D.C., Colombia;4. Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (MNCN-CSIC), José Gutiérrez Abascal 2, 28006 Madrid, Spain;5. Pathology Department, University of Texas Medical Branch, 301 University Blvd., Galveston, Texas, 77555, USA;6. Centro de Museos, Museo de Historia Natural, Universidad de Caldas, Calle 58 No. 21-50, 170004, Manizales, Caldas, Colombia |
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| Abstract: | The aim of this study was to evaluate tick fauna and identify the possible presence of Rickettsia spp. in ticks of the genus Amblyomma from two environmental preservation areas in different regions of Mato Grosso do Sul state, Brazil. CO2 traps, visual observation and cloth dragging were used to capture ticks. Three hundred ticks were submitted to the hemolymph test, and samples that showed organisms morphologically compatible with Rickettsia were used for rickettsial DNA detection by PCR. DNA was extracted using guanidine-phenol isothiocyanate, and the primers CS78 and CS323 were used for PCR, which amplified a 401-base pair fragment of the citrate synthase (gltA) gene. If positive, the DNA sample was tested by primers Rr190.70p and Rr190.602n that produce a 530 bp amplicon of the ompA gene that is present only in rickettsiae of the spotted fever group. A total of 1,745 adult ticks were collected, including 1,673 specimens of Amblyomma sculptum, 63 of Amblyomma coelebs, five of Amblyomma naponense and four of Amblyomma ovale. Thirteen ticks of the species A. ovale, A. coelebs and A. sculptum showed structures compatible with Rickettsia inside the hemocytes; after DNA extraction, the presence of Rickettsia spp. in a sample of A. ovale was confirmed by PCR in both analyzed fragments. In the sequencing analysis, 100% identity for the Rickettsia parkeri strain Atlantic rainforest was obtained according to GenBank. The two environmental preservation areas showed A. sculptum as the predominant species, as well as the presence of marked seasonality for this species. This paper is the first report of the R. parkeri strain Atlantic rainforest in A. ovale ticks in the state of Mato Grosso do Sul, Brazil. |
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