| Inhibitory Effect of Matrine on the Expression of PSA and AR in Prostate Cancer Cell line LNCaP |
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| 引用本文： | 谌科,胡志全,王涛,郭辉,叶章群. Inhibitory Effect of Matrine on the Expression of PSA and AR in Prostate Cancer Cell line LNCaP[J]. 华中科技大学学报(医学英德文版), 2008, 28(6): 697-699. DOI: 10.1007/s11596-008-0619-2 |
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| 作者姓名： | 谌科 胡志全 王涛 郭辉 叶章群 |
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| 作者单位： | Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology |
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| 摘 要： | In order to investigate the inhibitory effect of matrine on the expression of prostate specific antigen （PSA） and androgen receptor （AR） in prostate cancer cell line LNCaP in vitro, LNCaP cells were treated with matrine at different concentrations （0.5, 1.0, 1.5, 2.0 g/L） for 12-36 h. The growth activities of cancer cells were determined by MTT colorimetric assay. The AR level was measured by Western blotting. The expression of PSA was detected by using AXSYM system-chemical luciferase methods. The results showed that matrine could effectively inhibit the growth of androgen-dependent prostate cancer cell line LNCaP in vitro in a time-and dose-dependent manner （P〈0.05）. It could obviously decrease the level of AR （P〈0.01） and inhibit the expression of PSA in a dose-dependent manner （P〈0.05） in LNCaP cells. It was concluded that matrine could significantly suppress the growth of LNCaP cells and inhibit the expression of PSA and AR of prostate cancer cells.
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| 关 键 词： | 前列腺特异性抗原 LNCaP细胞 癌细胞系 抑制作用 苦参碱 AR 前列腺特异抗原 AXSYM系统 |
| 收稿时间： | 2007-12-14 |
Inhibitory effect of matrine on the expression of PSA and AR in prostate cancer cell line LNCaP |
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| Ke Chen,Zhiquan Hu,Tao Wang,Hui Guo,Zhangqun Ye. Inhibitory effect of matrine on the expression of PSA and AR in prostate cancer cell line LNCaP[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(6): 697-699. DOI: 10.1007/s11596-008-0619-2 |
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| Authors: | Ke Chen Zhiquan Hu Tao Wang Hui Guo Zhangqun Ye |
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| Affiliation: | Department of Urology, Tongfi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China |
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| Abstract: | Summary In order to investigate the inhibitory effect of matrine on the expression of prostate specific antigen (PSA) and androgen receptor (AR) in prostate cancer cell line LNCaP in vitro, LNCaP cells were treated with matrine at different concentrations (0.5, 1.0, 1.5, 2.0 g/L) for 12–36 h. The growth activities of cancer cells were determined by MTT colorimetric assay. The AR level was measured by Western blotting. The expression of PSA was detected by using AXSYM system-chemical luciferase methods. The results showed that matrine could effectively inhibit the growth of androgen-dependent prostate cancer cell line LNCaP in vitro in a time- and dose-dependent manner (P<0.05). It could obviously decrease the level of AR (P<0.01) and inhibit the expression of PSA in a dose-dependent manner (P<0.05) in LNCaP cells. It was concluded that matrine could significantly suppress the growth of LNCaP cells and inhibit the expression of PSA and AR of prostate cancer cells. Ke CHEN, male, born in 1979, Doctorial Candidate |
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| Keywords: | prostate cancer matrine LNCaP cells androgen receptor prostate specific antigen |
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