周围神经损伤后Tropic 1808基因表达蛋白的免疫组织化学研究

目的 研究Tropic 1808基因表达蛋白在受损大鼠坐骨神经中的表达与分布。方法 用酶联间接法和双重免疫荧光法进行免疫组织化学染色。并通过计算机图像处理技术，测量阳性区域的强度与面积。结果 损伤神经的近侧端和远侧端中施万细胞均有Tropic 1808基因表达蛋白的阳性免疫反应，阳性免疫反应物主要分布于施万细胞膜上，远侧端的阳性反应物强度和面积强于和大于近侧端。结论 周围神经损伤后，Tropic 1808基因表达蛋白分布于损伤近侧端和远侧端的施万细胞膜上，并且远侧端中该蛋白的表达强于近侧端。

AN IMMUNOHISTOCHEMICAL STUDY ON TROPIC 1808 GENE EXPRESSIONAL PROTEIN IN THE LESIONED SCIATIC NERVE

Objective To study the expression and the distribution of Tropic 1808 gene expressional protein in the lesioned sciatic nerves of adult SD rats. Methods Both distal and proximal segments of 12 days cut-off sciatic nerves of SD rats were obtained.Then immunohistochemistry and dual fluorescent-immunohistochemistry were performed to observe the expression of Tropic 1808 gene expressional protein in the lesioned sciatic nerves.The positive intensity and area of immuno-response were detected by the image analyses system. Results The Schwann cells's membranes which in the distal and proximal of lesioned sciatic nerves appeared to have positive expression of Tropic 1808 gene expressional protein.The positive intensity and area of immuno-response in the distal end were denser and larger than those in the proximal.Conclusion Tropic1808 gene expressional protein expresses in the Schwann cell's membranes of the distal and the proximal after the peripheral nerves were injured.The positive expression in the distal was higher than that in the proximal.;