| 人前列腺癌相关基因pc—1表达产物在细胞内的定位研究 |
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| 引用本文： | 张浩,周建光,孙玉龙,李杰之,袁兰,黄翠芬.人前列腺癌相关基因pc—1表达产物在细胞内的定位研究[J].军事医学科学院院刊,2001,25(4):255-259,264. |
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| 作者姓名： | 张浩 周建光 孙玉龙 李杰之 袁兰 黄翠芬 |
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| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100850
2. 北京大学医学部医药卫生分析中心,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 0 70 2 96 ) |
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| 摘 要： | 目的：研究pc-l基因表达产物在细胞内的定位。方法：采用巢式PCR技术，从原始克隆质粒中扩增出可编码蛋白分子的pc-l cDNA片段，分别将其克隆入含EGFP和MYC标签的真核表达载体中；用脂质体介导法将其转染入人前列腺癌细胞C4－2中；通过免疫细胞化学方法和激光共聚焦显微镜技术检测pc-l表达产物在细胞内的定位。结果与结论：pc-l表达产物定位于细胞质中，为进一步研究其生理、生化功能奠定了基础。
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| 关 键 词： | 前列腺肿瘤 pc－l 免疫细胞化学 激光共聚焦 细胞定位 |
| 文章编号： | 1000-5501(2001)04-0255-06 |
Localization of the expression product of human prostatic cancer associated pc-1 gene in cells |
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| ZHANG Hao ,ZHOU Jian Guang ,SUN Yu Long ,LI Jie Zhi ,YUAN Lan ,HUANG Cui Fen.Localization of the expression product of human prostatic cancer associated pc-1 gene in cells[J].Bulletin of the Academy of Military Medical Sciences,2001,25(4):255-259,264. |
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| Authors: | ZHANG Hao ZHOU Jian Guang SUN Yu Long LI Jie Zhi YUAN Lan HUANG Cui Fen |
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| Institution: | ZHANG Hao 1,ZHOU Jian Guang 1,SUN Yu Long 1,LI Jie Zhi 1,YUAN Lan 2,HUANG Cui Fen 1 |
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| Abstract: | Objective:To investigate the localization of the expression product of pc 1 gene in cells. Methods: The protein coding region of pc 1 cDNA was amplified using nested PCR technology from the primary cloning plasmid and subcloned into the eukaryotic expressing vectors pEGFP N1 and pCDNA3.1( )/myc his containing EGFP and MYC tag,respectively,then transfected into the human prostatic cancer cell line C4 2.The location of its expression product in C4 2 was observed with immunocytochemistry and laser confocal scanning microscopy. Results and Conclusions:The expression product of pc 1 gene located in the cytoplasm. This work provides the basis for further study of the biochemical and physiological functions of the pc 1 gene. |
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| Keywords: | prostatic neoplasm pc 1 immunocytochemistry laser confocal scanning cellular location |
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