| 正交试验法优选桔梗非药用部位多糖的提取工艺 |
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| 引用本文： | 于九洋,段元静,陈 波,顾伟杰,华 芳,桂双英.正交试验法优选桔梗非药用部位多糖的提取工艺[J].安徽中医学院学报,2012,31(5):59-60. |
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| 作者姓名： | 于九洋 段元静 陈 波 顾伟杰 华 芳 桂双英 |
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| 作者单位： | [1]安徽中医学院药学院,安徽合肥230031 [2]安徽省中药制剂工程技术研究中心,安徽合肥230031 [3]现代中药安徽省重点实验室,安徽合肥230031 |
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| 基金项目： | “十二五”国家科技支撑计划项目（2011BA104806）;安徽中医学院药学院药苑之星科研项目（2011012）;安徽省自然科学基金项目（11040606M219）;安徽高校省级自然科学研究重点项目（KJ2012A184） |
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| 摘 要： | 目的优选桔梗非药用部位多糖的最佳提取工艺。方法以桔梗多糖得率为指标,采用正交设计法考察料液比、浸提次数、浸提时间对提取工艺的影响,以确定最佳提取工艺。结果最佳提取工艺参数为:加25倍量水,提取3次,每次1h。结论优选的提取工艺稳定、可行。
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| 关 键 词： | 桔梗 桔梗多糖 正交试验 提取工艺 |
Orthogonal Design-Based Optimization of Extraction Technology of Total Polysaccharides from Non-Medicinal Parts of Platycodon Grandiforus |
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| YU Jiu-yang,DUAN Yuan-jing,CHEN Bo,GU Wei-jie,HUA Fang,GUI Shuang-ying.Orthogonal Design-Based Optimization of Extraction Technology of Total Polysaccharides from Non-Medicinal Parts of Platycodon Grandiforus[J].Journal of Anhui Traditional Chinese Medical College,2012,31(5):59-60. |
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| Authors: | YU Jiu-yang DUAN Yuan-jing CHEN Bo GU Wei-jie HUA Fang GUI Shuang-ying |
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| Institution: | 1. School of Pharmacy, Anhui College of Traditional Chinese Medicine, Anhui Hef ei 230031, China ; 2. Anhui Engineering Research Center for Modern Chinese Materia Medica, Anhui Hefei 230031, China; 3. Anhui Key Laboratory .for Modern Chinese Materia Medica, Anhui Hefei 230031, China) |
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| Abstract: | Objective To optimize the extraction technology of total polysaccharides from the non-medicinal parts of Platycodon grandiforus. Methods With extraction rate yield of total polysaccharides as the indica-tor, an orthogonal design was used to investigate the effects of material/water ratio, extraction times, and extraction time on extraction process and to determine the optimal extraction process. Results The opti-mum extraction parameters were as follows： material/water ratio of 1：25, 3 times of extraction, and 1 h each time of extraction. COnclusion The optimized technology for extraction of total polysaccharides from the non-medicinal parts of Platycodon grandiforus is stable, reasonable, and feasible. |
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| Keywords: | Radix Platycodonis polysaecharides of Radix Platycodonis orthogonal design extractiontechnology |
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