须根水提液对白花丹参种子萌发幼苗光合作用及次生代谢的影响

目的:研究不同浓度白花丹参须根水提液对种子萌发、幼苗光合作用及次生代谢物质积累的影响,以期为白花丹参连作障碍的消减提供参考。方法:设置不同浓度白花丹参须根水提液处理组,测定不同处理组种子萌发率、幼苗多酚氧化酶活性、可溶性糖及可溶性蛋白含量;利用高效液相色谱法(HPLC)测定幼苗中水溶性及脂溶性有效物质含量。结果:白花丹参须根水提液低浓度对种子萌发有促进作用,高浓度有抑制作用;与对照组相比,处理组幼苗净光合速率、蒸腾速率、气孔导度均有不同程度下降;对照组多酚氧化酶活性及可溶性蛋白、可溶性糖总量分别比白花丹参须根水提液处理后低7.86%、8.47%、14.33%,但处理后幼苗水溶性有效成分(丹酚酸B、迷迭香酸)及脂溶性有效成分(二氢丹参酮、丹参酮Ⅰ、丹参酮Ⅱ_A、隐丹参酮)含量积累量均高于对照组。结论:白花丹参须根水提液通过影响种子的萌发率、抑制幼苗的光合作用、促进次生代谢产物积累影响丹参的产量及品质。

Effects of Water Extract of Fibrous Root on Seed Germination,Photosynthesis and Secondary Metabolism of Salvia miltiorrhiza

Objective:In order to provide basic data for reducing the continuous cropping obstacle of Salvia miltiorrhiza Bge.F.alba,the effects of different concentrations of fibrous root water extract on seed germination,seedling photosynthesis and secondary metabolite accumulation were studied.Methods:Germination rate of seeds,polyphenol oxidase activity,soluble sugar and protein content were measured in different treatment groups,and also the content of water-soluble and lipid soluble effective substances in S.miltiorrhiza seedlings were determined by HPLC.Results:Compared with the control group,the net photosynthetic rate(P n),transpiration rate(T r)and stomatal conductance(G s)of the treated group are decreased.The PPO activity,the content of soluble protein and total soluble sugar in the control group were 7.86%,8.47%and 14.33%lower than those in the fibrous root water extract group,respectively,but the accumulation of water-soluble(salvianolic acid B,rosmarinic acid)and lipid soluble effective substance(dihydrotanshinone,tanshinoneⅠ,tanshinoneⅡ_A,cryptotanshinone)in the treated seedlings were higher than that in the control group.Conclusion:The water extract of fibrous root affected the germination rate of seeds,inhibited photosynthesis of seedlings,promoted the accumulation of secondary metabolites,and affected the yield and quality of S.miltiorrhiza.