4种9,10-二氢菲类化合物的体外抗肿瘤活性及机制

 目的研究4种9,10-二氢菲类化合物对4种人癌细胞的增殖抑制作用及其作用机制。方法采用MTT法测定它们对人肝癌细胞株HepG2,人非小细胞肺癌细胞株NCI-H460,人乳腺癌细胞株MCF-7,人神经胶质瘤细胞株SF-268的增殖抑制作用;采用流式细胞仪技术和蛋白质电泳技术检测它们对HepG2细胞的细胞周期以及细胞周期蛋白表达的影响。结果9,10-二氢菲类化合物对HepG2细胞的增殖有一定的抑制作用;其中4,5-二羟基-2-甲氧基-9,10-二氢菲可能通过下调周期蛋白CyclinB1的表达将HepG2细胞阻滞在G2/M期。结论9,10-二氢菲类化合物通过将HepG2细胞阻滞在G2/M期从而对其增殖产生了一定的抑制作用。

Study on Cytotoxicity of Four 9,10-Dihydrophenanthrenes

OBJECTIVE To investigate the anti-proliferative effect of four 9, 10-dihydrophenanthrenes isolated from Pholidota yunnanesis Rolfe on 4 human carcinomatous cells and the mechanism of the induced cytotoxicity. METHODS Growth inhibition by the four compounds was analyzed using MTT assay. The effect on the cell cycle of HepG2 cells was detected using flow cytometry. The protein expression was determined using Western blot. RESULTS 9,10-dihydrophenanthrenes showed considerable cytotoxic activity against HepG2 cells; 4, 5-dihydroxy-2-methoxy-9, 10-dihydrophenanthrene treatment induced the accumulation of cells in the G2/M phase and the protein level of Cdc-2 was not changed but that of Cyclin B1 was decreased in the treated cells. CONCLUSION 9,10- Dihydrophenanthrenes show cytotoxic activity against HepG2 cells by arresting the cell at G2/M phase.