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Monitoring of Patients Supported by Extracorporeal Membrane Oxygenation for Systemic Infections by Broad-Range rRNA Gene PCR Amplification and Sequence Analysis
Authors:Peter Orszag  Claudia Disqué   Sonja Keim  Michael G. Lorenz  Olaf Wiesner  Johannes Hadem  Meike Stiesch  Axel Haverich  Christian Kühn
Affiliation:aDepartment of Thoracic, Transplant, and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany;bMolzym GmbH & Co. KG, Bremen, Germany;cDepartment of Respiratory Medicine, Hannover Medical School, Hannover, Germany;dDepartment of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany;eDepartment of Prosthetic Dentistry, Hannover Medical School, Hannover, Germany
Abstract:The rRNA gene PCR and sequencing test, SepsiTest, was compared with blood culture (BC) regarding the diagnosis of pathogens in 160 blood samples drawn from 28 patients during extracorporeal membrane oxygenation. With 45% of positive samples, SepsiTest was 13 to 75 h faster than BC. SepsiTest indicated bacteremias in 25% of patients who were BC negative.
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