四逆散冻干粉对睡眠剥夺果蝇头部5-HT含量和5-HT_(1A)受体表达的影响

目的:探讨四逆散冻干粉改善果蝇睡眠作用的机制。方法:收集12 h内羽化未交配的野生型Canton S品系黑腹果蝇,小气流CO2麻醉,雌雄分开,雌雄分别随机分为空白对照组、睡眠剥夺模型组和给药组,每组30只果蝇,利用ELISA法分别测定各组果蝇头部的5-HT的含量。利用实时定量PCR法,测定各组果蝇头部5-HT1A受体的表达差异。结果:四逆散冻干粉分别能提高雌性与雄性果蝇头部5-HT的含量,与模型组相比具有极显著性差异(P0.01)。四逆散冻干粉还能增加果蝇头部5-HT1A受体的表达量。结论:四逆散冻干粉改善果蝇睡眠的作用机制与增加果蝇头部5-HT含量和促进果蝇头部5-HT1A受体表达有关。

Effect of lyophilized powder of Sini powder on 5-HT content and 5-HT1A receptor in drosophila heads

Objective : To unveil the mechanism of sleep-improving effect of lyophilized powder of Sini powder on drosophila. Method : Drosophila melanogaster whose strain were wild Canton S, unmated, were collected during 12 h after their emergencing, then they were anaesthetized by small flow of CO₂ and divided into different tubes due to their sex. Both male and female flies were distributed randomly into three groups as blank,control and administration, 32 flies each group. The content of 5-HT in the brain was estimated was estimated with ELISA and had a comparison of the expression of 5-HT_1A receptor in brain, using real-time quantitative PCR. Result : Concentration of 5-HT in brain was increased in administration group, and had a significant difference to control group. The expression of 5-HT_1A receptor was also up-regulated after administrated of Sini powder. Conclusion : Sleep-improving effect of Sini powder on drosophila has a contribution to the increasing of 5-HT and 5-HT_1A receptor in brain.