转化生长因子β_1、白细胞介素2、7与卵巢上皮性癌患者腹腔局部免疫功能的关系

目的:探讨转化生长因子β1(TGFβ1)和白细胞介素-2(IL-2)、白细胞介素-7(IL-7)与卵巢上皮性癌腹腔局部免疫功能的关系。方法:采用酶联免疫吸附试验(ELISA)法,测定49例卵巢上皮性癌,18例卵巢良性上皮性肿瘤患者血清、腹腔液中TGFβ1和IL-2、IL-7水平,比较两组之间各细胞因子水平的差异性及其相互关系。结果:卵巢上皮性癌患者血清中TGFβ1、IL-2水平与良性对照组差异无显著性(P>0.05),IL-7水平则高于对照组(P<0.05);腹腔液中TGFβ1水平明显高于对照组(P<0.01),IL-2水平明显低于对照组(P<0.05),IL-7水平则与对照组相比差异无显著性(P>0.05)。Ⅲ期患者腹腔液中TGFβ1水平明显高于Ⅰ、Ⅱ期(P<0.01)。卵巢上皮性癌患者血清与腹腔液中TGFβ1、IL-2相关性不显著,而IL-7水平存在正相关;卵巢上皮性癌患者血清中TGFβ1;、IL-2和IL-7水平两两之间均无明显相关性;但腹腔液中TGFβ1与IL-2、IL-7水平均存在显著的负相关,IL-2与IL-7水平则无明显相关性。结论:卵巢癌存在腹腔局部免疫缺陷,腹腔液中TGFβ1、IL-2和IL-7参与这一过程,且3者之间可能存在一定的关系。

Transforming growth factor-β1,interleukin-2,interleukin-7 and their correlation with local immunity in peritoneal cavity of the patients with ovarian carcinoma

Objective: To evaluate the correlation between transforming growth factor β1 (TGFβ), interleukin-2 (IL-2), interleukin-7 (IL-7) level in serum and ascites and local immunity in peritoneal cavity of the patients with ovarian epithelial carcinoma. Methods: TGFβ1, IL-2, IL-7 level in serum and ascitics of 49 patients with ovarian epithelial carcinoma were measured by enzyme-liked immunosorbent assay (ELISA). 18 patients with ovarian benign epithelial tumor were collected as a control group. Results: TGFβ1 and IL-2 levels in serum were not significantly different between ovarian carcinoma and control group, but IL-7 serum level of ovarian carcinoma group was significantly elevated than that of control group (P<0.05). Compared with the control group, TGFβ1 ascites level was increased but IL-2 level decreased significantly (P<0.01, P < 0.05 respectively) in ovarian carcinoma group. In ovarian carcinoma group, there were no correlations of TGFβ1 and IL-2 level between in serum and in ascitis, but postive correlations of IL-7 level existed. Furthermore, there was no correlation among the TGFβ1, IL-2 and IL-7 serum levels, but TGFβ1 in level ascite was negatively correlated with IL-2 and IL-7 levels in ascites, respectively. Conclusions: Immunosuppression may exist in peritoneal cavity of the patients with ovarian epithelial carci-noma. TGFβ1, IL-2 and IL-7 in ascitis maybe correlated with each other and play a role in this im-munosuDDressive status.