3-硝基丙酸对沙土鼠海马CA1区锥体细胞超微结构的影响

目的观察小剂量3-硝基丙酸(≤20mg/kg)对沙土鼠海马CA1区锥体细胞超微结构的影响.方法将沙土鼠腹腔注射不同剂量3-硝基丙酸或双蒸水后3d,取海马CA1 区用戊二醛和锇酸固定,电镜下观察CA1区锥体细胞层神经元细胞核和细胞器超微结构形态变化,用体视学方法计算每张照片线粒体和粗面内质网体面积变化.结果实验组与对照组比较细胞核无明显变化,线粒体和粗面内质网轻度肿胀,体密度高于对照组.结论小剂量3-硝基丙酸可引起神经元缺氧代偿性反应,无致死性损害.

Influence of 3-nitropropionic acid on ultrastructural changes of hippocampal CA1 region neurons in gerbils

OBJECTIVE To investigate influence of 3-nitropropionic acid(3-NPA) on ultrastructural changes of hippocampal CA 1 region neurons in gerbils. METHOD Gerbils were randomly divided into control group and experimental groups, 3-NPA and distilled water were administered by intraperitoneal injection respectively. Three days after the introperitoneal injection, the CA 1 pyramidal neurons were studied by electron microscope, morphological observation and volume density of mitochondrion and rough endoplasmic reticulum were made.RESULTS Nucleus changes of CA 1 pyramidal neurons in the experimental groups were not significant differences compared with the control group, ultrostructural changes occurred in mitochondrion and rough endoplasmic reticulum.CONCLUSION Low dose of 3-NPA might induce compensable reaction, but not lead to deadly injury.