氟哌啶醇对氯胺酮诱导的热休克蛋白70在大鼠海马中表达的影响

目的 研究氟哌啶醇对于氯胺酮诱导的热休克蛋白70（HSP70）在大鼠海马中表达的影响，探讨氟哌啶醇能否预防或治疗氯胺酮导致的脑损害。方法 选择48只大鼠，随机分为8组，分别在腹腔注射氯胺酮80．0mg/kg前后给予不同剂量氟哌啶醇（1．0，5．0，10．0mg/kg),24h后取鼠脑，应用HSP70单克隆抗体免疫组化染色检测大鼠海马中HSP70的表达，并用MIAS－2000图文分析系统分析大鼠海马HSP70阳性细胞百分率、阳性细胞密度和灰度值。结果 单独应用氯胺酮可在大鼠海马表达HSP70；预先给予氟哌啶醇能抑制氯胺酮诱导的HSP70表达，且随着剂量的增加，其抑制作用增强；在使用氯胺酮后给予氟哌啶醇，不能减少HSP70的表达。结论 氯胺酮可诱导HSP70在海马的表达，提示海马的神经元可能受到损害；氟哌啶醇可以预防氯胺酮所致的神经元损害，但对已造成的神经元损害无治疗作用。

Effects of haloperidol on the expression of HSP70 induced by ketamine in the rat hippocampus

Objective This experiment was designed to determine the effect of haloperidol on HSP70 induced by ketamine, and to explore the possibility of using haloperidol to prevent or treat the brain injury caused by ketamine.Methods 48 rats were divided into 8 groups, In group 1-3 different doses of haloperidol (1.0,5.0,10.0 mg/kg)were given 1h before the administration of ketamine. There were two control groups. In control group 1 normal saline was given twice with 1h interval between the two injections. In control group 2 ketamine 80.0 mg/kg was given 1h after the administration of normal saline.The volume of each injection was 3ml and intraabdominal injection was used as the route of administration.HSP70 mono-clone antibody immunocytochemistry was used to detect HSP70 expression in rat hippocampus, and MIAS-2000 photography analytic software to analyze HSP70 expression in hippocampus of the rat brain.Results Ketamine induced HSP70 expression in the rat brain. Pretreatment with haloperidol inhibited HSP70 expression induced by ketamine, and the inhibition was dose-dependent, but haloperidol given after the administration of ketamine could not decrease HSP70 expression.Conclusions Ketamine injures neurons of rat hippocampus and induces the expression of HSP70 and haloperidol pretreatment can prevent neuronal injury caused by ketamine, but haloperidol can not antagonize the injury caused by ketamine.