用溴甲酚绿作利血平的比色测定

本文就二十多种染料中,筛选了溴甲酚绿、猩红磺酸盐、溴甲酚红紫、溴麝香草酚蓝及橙黄Ⅱ等作显色剂。各该染料与利血平在一定pH缓冲溶液的影响下,均可形成复盐,在氯仿中呈现不同颜色,并具有特征吸收光谱。文內拟定了用溴甲酚绿作利血平比色测定的方法,应用于制剂分析,结果良好。

Adaptation of Bromcresol Green Method for the Determination of Reserpine

A series of dyes were selected for experimentation as reagents for the colorimetric determination of reserpine. Among these, bromcresol green, scaret red sulfonate, bromthymol blue, bromcresol purple and orange Ⅱ when combined with reserpine at a certain pH (phosphate-citrate buffer), formed sensitive and stable colored addition products in chloroform solution. These have not hitherto been reported in the literature. The optimum conditions for the reaction of reserpine with bromcresol green have been studied in detail. A method which obeys Beer's law, is proposed giving a suitable concentration of reserpine, ranging from 2 to 23 mcg per ml of chloroform at pH 3±0.5.The extinction was measured colorimetrically 420 mμ against a reagent blank, and was shown to remain stable for eight hours at room temperature. Results agree well with those obtained by the USP ⅩⅥ method.This method provides a more rapid, convenient, accurate and simple process for the colorimetric determination of reserpine in pharmaceutical preparations. It also gives a mean deviation of <±2% from the average.