茶多酚对同型半胱氨酸诱导损伤的人血管内皮细胞纤溶功能的影响

目的研究茶多酚(TP)对同型半胱氨酸(Hcy)损伤的人脐静脉内皮细胞(HUVEC)形态及组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂-1(PAI-1)含量的影响。方法将HUVEC分为同时给予TP和Hcy组与先给予Hcy再给予TP组,各组又按TP浓度不同分成若干小组,根据细胞形态变化和ELISA法测定细胞上清液中t-PA和PAI-1的含量变化。结果无论同时给予TP和Hcy组还是先给予Hcy再给予TP组,中浓度(4、8μg/ml)和高浓度(16μg/ml)TP组细胞形态较单纯Hcy组细胞形态规则,立体感增强,胞浆内颗粒减少。ELISA结果显示,单纯Hcy组与正常对照组相比,PAI-1含量增加(P<0.05),含中、高浓度TP组,PAI-1含量较单纯Hcy组降低,且与TP浓度呈剂量依赖性降低(P<0.05),低浓度TP组PAI-1含量与单纯Hcy组差异无显著性;各组t-PA差异均无显著性。结论一定浓度的TP可以恢复Hcy引起的HUVEC细胞形态改变,且可以逆转Hcy引起的PAI-1含量增高的现象。

Influence of tea polyphenols on the damage of fibrinolytic system induced by homocysteine in human umbilical vein endothelial cells

Objective To study the influence of tea polyphenols(TP) on cells form and fibrinolytic system which focused on tissue-type plasminogen activator(t-PA) and plasminogen activator inhibitor-1(PAI-1) induced by homocysteine(Hcy) in human umbilical vein endothelial cells(HUVEC).Methods HUVEC were divided into two groups,one was administered with TP and Hcy at the same time,the other was firstly treated with Hcy for 12 hours and followed by administering TP. Each group was divided into some groups depending on different concentrations of TP. The t-PA and PAI-1 levels of HUVEC supernatant were determined by ELISA assay.Results Compared with the groups that just treated with Hcy,medium concentration (4 and 8μg/ml) and high concentration (16μg/ml) of TP could strengthen cells stereoscopic effect and decrease endochylema particles; the results of ELISA demonstrated that the concentrations of PAI-1 were increased in the groups that just treated with Hcy compared with control group(P<0.05),administrations of medium and high dose of TP could decrease the concentration of PAI-1,and the concentration of PAI-1 were decreased depending on the increase of TP concentration(P<0.05),there was no difference between low concentration TP group and the group only treated with Hcy. There were no differences on the concentrations of t-PA among each groups.Conclusion The proper concentration of TP could restore the damage of HUVEC form and decrease the concentration of PAI-1 induced by Hcy.